Ayehunie S, Belay A, Baba T W, Ruprecht R M
Laboratory of Viral Pathogenesis, Dana-Farber Cancer Institute, and Harvard Medical School, Boston, Massachusetts 02115, USA.
J Acquir Immune Defic Syndr Hum Retrovirol. 1998 May 1;18(1):7-12. doi: 10.1097/00042560-199805010-00002.
An aqueous extract of the blue-green filamentous algae Arthrospira platensis (previously called Spirulina platensis) inhibited HIV-1 replication in human T-cell lines, peripheral blood mononuclear cells (PBMC), and Langerhans cells (LC). Extract concentrations ranging between 0.3 and 1.2 microg/ml reduced viral production by approximately 50% (50% effective concentration [EC50]) in PBMCs. The 50% inhibitory concentration (IC50) of extract for PBMC growth ranged between 0.8 and 3.1 mg/ml. Depending on the cell type used, therapeutic indices ranged between 200 and 6000. The extract inactivated HIV-1 infectivity directly when preincubated with virus before addition to human T-cell lines. Fractionation of the extract revealed antiviral activity in the polysaccharide fraction and also in a fraction depleted of polysaccharides and tannins. We conclude that aqueous A platensis extracts contain antiretroviral activity that may be of potential clinical interest.
蓝绿色丝状藻类钝顶节旋藻（以前称为钝顶螺旋藻）的水提取物可抑制HIV-1在人T细胞系、外周血单核细胞（PBMC）和朗格汉斯细胞（LC）中的复制。提取物浓度在0.3至1.2微克/毫升之间时，可使PBMC中的病毒产生减少约50%（50%有效浓度[EC50]）。提取物对PBMC生长的50%抑制浓度（IC50）在0.8至3.1毫克/毫升之间。根据所使用的细胞类型，治疗指数在200至6000之间。在添加到人类T细胞系之前，提取物与病毒预孵育时可直接使HIV-1失活。提取物的分级分离显示多糖部分以及不含多糖和单宁的部分具有抗病毒活性。我们得出结论，钝顶节旋藻水提取物含有抗逆转录病毒活性，可能具有潜在的临床意义。
