Uochi T, Asashima M
Department of Life Science (Biology), The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8912, Japan.
Gene. 1998 May 12;211(2):245-50. doi: 10.1016/s0378-1119(98)00102-4.
The pronephros functions in the amphibian larval stage. It differentiates in certain presumptive regions of the amphibian embryo. The study of molecules functioning during pronephrogenesis is important for understanding the mechanism of kidney formation. Herein, we report a gene expressed during differentiation of the pronephros and neural tissues that we isolated by differential hybridization using our pronephros in-vitro induction system. The gene, XCIRP, is 887bp in length, and encodes a putative protein composed of 163 amino acid residues. The deduced protein contains two CS-RBDs (consensus sequence RNA-binding domain) and a glycine-rich domain, and is 74% identical to homologs from other species (mouse, rat and human). The expression of XCIRP increased rapidly during gastrulation, and XCIRP localization was seen in the presumptive pronephros and neural tissues. These findings suggest that XCIRP may play important roles in pronephrogenesis and neurogenesis.
前肾在两栖类幼体阶段发挥功能。它在两栖类胚胎的某些预定区域分化。研究前肾发生过程中发挥作用的分子对于理解肾脏形成机制很重要。在此，我们报告一个在前肾和神经组织分化过程中表达的基因，该基因是我们利用前肾体外诱导系统通过差异杂交分离得到的。这个基因，XCIRP，长度为887bp，编码一个由163个氨基酸残基组成的假定蛋白质。推导的蛋白质包含两个CS-RBDs（共有序列RNA结合结构域）和一个富含甘氨酸的结构域，与其他物种（小鼠、大鼠和人类）的同源物有74%的同一性。XCIRP的表达在原肠胚形成期间迅速增加，并且在预定的前肾和神经组织中可见XCIRP定位。这些发现表明XCIRP可能在前肾发生和神经发生中发挥重要作用。
