Yawalkar N, Brand C U, Braathen L R
Institute of Immunology and Allergology, University of Bern, Inselspital, Switzerland.
Br J Dermatol. 1998 Feb;138(2):297-300. doi: 10.1046/j.1365-2133.1998.02078.x.
Recent reports suggest that production of interleukin-12 (IL-12) by dendritic cells and keratinocytes may play an important part in contact hypersensitivity reactions. In the present study we investigated mRNA and protein expression of IL-12 in human skin lymph derived from normal untreated skin (n = 5) and from the induction phase of allergic contact dermatitis (CD) (n = 5). mRNA levels were determined at various time points in the lymph cells by a nested reverse transcriptase-polymerase chain reaction method. Time course analysis reproducibly revealed a constitutive expression of both IL-12 p40 and p35 mRNA in the migrating lymph cells in all volunteers. However, no enhancement of the IL-12 mRNA signal was found during the induction phase of allergic CD. Furthermore, as determined by a sensitive ELISA technique, IL-12 protein was not detectable in 60 lymph samples derived from normal untreated skin or in 68 lymph samples obtained during the induction phase of allergic CD at any time point of the lymph cannulation. In conclusion, our findings indicate that no significant protein levels of IL-12 are washed out from the skin into the afferent lymph or are produced and released by migrating lymph cells during the induction phase of allergic CD in vivo.
最近的报告表明，树突状细胞和角质形成细胞产生的白细胞介素-12（IL-12）可能在接触性超敏反应中起重要作用。在本研究中，我们调查了来自正常未处理皮肤（n = 5）和过敏性接触性皮炎（CD）诱导期（n = 5）的人皮肤淋巴液中IL-12的mRNA和蛋白质表达。通过巢式逆转录聚合酶链反应方法在淋巴细胞的不同时间点测定mRNA水平。时间进程分析可重复地显示所有志愿者迁移淋巴细胞中IL-12 p-40和p-35 mRNA的组成性表达。然而，在过敏性CD的诱导期未发现IL-12 mRNA信号增强。此外，通过敏感的ELISA技术测定，在来自正常未处理皮肤的60份淋巴液样本中或在过敏性CD诱导期获得的68份淋巴液样本中的任何时间点均未检测到IL-12蛋白。总之，我们的研究结果表明，在体内过敏性CD诱导期，没有明显水平的IL-12蛋白从皮肤冲洗到传入淋巴液中，也没有由迁移的淋巴细胞产生和释放。
