Moka D, Vorreuther R, Schicha H, Spraul M, Humpfer E, Lipinski M, Foxall P J, Nicholson J K, Lindon J C
Department of Nuclear Medicine, University of Cologne, Germany.
J Pharm Biomed Anal. 1998 May;17(1):125-32. doi: 10.1016/s0731-7085(97)00176-3.
High resolution 1H nuclear magnetic resonance (NMR) spectra using spinning at the magic angle (1H MAS NMR) have been obtained on intact normal and pathological kidney tissue samples from patients undergoing surgery for renal cell carcinoma (RCC). The spectra were measured on ca. 80 mg samples and provided high resolution 1H NMR spectra in which effects of dipolar couplings, chemical shift anisotropy and magnetic susceptibility differences are minimised thus yielding high spectral resolution. Conventional one-dimensional and spin-echo spectra and two-dimensional J-resolved, TOCSY and 1H-13C HMQC spectra were also measured on selected samples and these allowed the assignment of resonances of endogenous substances comprising both cytosolic and membrane components. The tumour tissues were characterised principally by an increased lipid content. These are the first reported results on human tumour tissues using this technique and the approach offers potential for the rapid classification of different types of tumour tissue.
利用魔角旋转的高分辨率氢核磁共振（NMR）光谱（1H MAS NMR）已在接受肾细胞癌（RCC）手术患者的完整正常和病理肾脏组织样本上获得。光谱是在约80毫克的样本上测量的，并提供了高分辨率的1H NMR光谱，其中偶极耦合、化学位移各向异性和磁化率差异的影响被最小化，从而产生高光谱分辨率。还对选定样本测量了传统的一维和自旋回波光谱以及二维J分辨、TOCSY和1H-13C HMQC光谱，这些光谱有助于确定包括胞质和膜成分在内的内源性物质的共振峰。肿瘤组织的主要特征是脂质含量增加。这是首次报道使用该技术对人类肿瘤组织的研究结果，该方法为快速分类不同类型的肿瘤组织提供了潜力。
