West N X, Maxwell A, Hughes J A, Parker D M, Newcombe R G, Addy M
Department of Restorative Dentistry (Periodontology), Bristol, UK.
J Dent. 1998 May;26(4):329-35. doi: 10.1016/s0300-5712(97)00025-0.
Acidic soft drinks are frequently implicated in dental erosion, but there are limited supporting data. Research is problematic due to the insidious nature of erosion and accuracy in assessing tissue loss. The aim of this study was to develop and validate, using a negative control, a model to accurately measure erosion in situ due to a single aetiological agent over a relatively short time period.
An intra-oral appliance capable of retaining an enamel sample was designed in order to assess the effect of orange juice consumption on enamel. The study was a single centre, randomized, placebo controlled, blind, crossover design.
Ten subjects, each consuming 11 of orange juice per day for 15 days, showed significantly more erosion on the enamel specimens than the same subjects consuming 11 of water per day over the same time period, measurements undertaken with surfometry. The same investigation was performed in vitro. Again, orange juice was significantly more erosive; indeed, it was in the order of 10 times that produced in situ. Surface microhardness testing in situ and in vitro demonstrated statistically significant differences between exposed and unexposed areas after orange juice treatment.
Changes produced by water either in situ or in vitro were always well within the baseline measurement parameters (+/- 0.3 micron) set down for the method and hence validated the clinical model in terms of reproducibility and accuracy in measurement. It is concluded that this method has confirmed the erosive potential of orange juice in situ. The method could have many applications to study dental erosion under highly controlled conditions and over realistic time periods.
酸性软饮料常被认为与牙侵蚀有关，但支持数据有限。由于侵蚀的隐匿性和评估组织损失的准确性，相关研究存在问题。本研究的目的是开发并验证一种模型，该模型使用阴性对照，能够在相对较短的时间段内准确测量由单一病因引起的原位侵蚀。
设计了一种能够固定牙釉质样本的口腔内装置，以评估饮用橙汁对牙釉质的影响。该研究为单中心、随机、安慰剂对照、盲法、交叉设计。
10名受试者每人每天饮用1升橙汁，持续15天，与同一时间段内每天饮用1升水的相同受试者相比，用表面光度测量法进行测量时，牙釉质标本上的侵蚀明显更多。在体外进行了相同的研究。同样，橙汁的侵蚀性明显更强；实际上，其侵蚀程度约为原位产生的10倍。原位和体外的表面显微硬度测试表明，橙汁处理后，暴露区域和未暴露区域之间存在统计学上的显著差异。
水在原位或体外产生的变化始终在该方法设定的基线测量参数（±0.3微米）范围内，因此在测量的可重复性和准确性方面验证了临床模型。得出的结论是，该方法已证实橙汁在原位具有侵蚀潜力。该方法可在高度受控的条件下和实际时间段内用于研究牙侵蚀，有许多应用价值。
