Jinbo T, Hayashi S, Iguchi K, Shimizu M, Matsumoto T, Naiki M, Yamamoto S
Department of Immunology, Faculty of Environmental and Health Sciences, Azabu University, Sagamihara, Kanagawa, Japan.
Vet Immunol Immunopathol. 1998 Feb 27;61(2-4):195-202. doi: 10.1016/s0165-2427(97)00148-7.
Monkey-specific C-reactive protein (CRP) assay methods (enzyme-linked immunosorbent assay (ELISA) and turbidimetric immunoassay (TIA)) were developed. The anti-monkey CRP serum was prepared by immunization of rabbits with the immune complex formed between the acute-phase serum from turpentine oil-inoculated monkeys and goat anti-human CRP serum. The specificity of the rabbit anti-monkey CRP serum was confirmed by immunoelectrophoresis and Western blotting. The purity of monkey CRP prepared by chromatography procedures was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The serum CRP levels in nine normal monkeys, as measured by sandwich ELISA were ranged from 0.26 to 1.42 microg/ml (mean 0.71+/-0.37). The CRP levels in five acute-phase sera of turpentine oil-inoculated monkeys were 248-451 microg/ml (mean 371.2+/-73.8). This monkey-specific CRP assay method was found more sensitive than the human-specific CRP assay method in detecting monkey CRP by TIA.
开发了猴特异性C反应蛋白（CRP）检测方法（酶联免疫吸附测定法（ELISA）和比浊免疫测定法（TIA））。用松节油接种猴的急性期血清与山羊抗人CRP血清形成的免疫复合物免疫兔，制备抗猴CRP血清。通过免疫电泳和蛋白质印迹法确认兔抗猴CRP血清的特异性。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）确认通过色谱法制备的猴CRP的纯度。通过夹心ELISA测定的9只正常猴的血清CRP水平范围为0.26至1.42微克/毫升（平均0.71±0.37）。松节油接种猴的5份急性期血清中的CRP水平为248 - 451微克/毫升（平均371.2±73.8）。发现在通过TIA检测猴CRP方面，这种猴特异性CRP检测方法比人特异性CRP检测方法更灵敏。
