Morgenstern B, Frech K, Dress A, Werner T
GSF-National Research Center for Environment and Health, Institute of Biomathematics and Biometry, Institute of Mammalian Genetics, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany.
Bioinformatics. 1998;14(3):290-4. doi: 10.1093/bioinformatics/14.3.290.
DIALIGN is a new method for pairwise as well as multiple alignment of nucleic acid and protein sequences. While standard alignment programs rely on comparing single residues and imposing gap penalties, DIALIGN constructs alignments by comparing whole segments of the sequences. No gap penalty is employed. This point of view is especially adequate if sequences are not globally related, but share only local similarities, as is the case in genomic DNA sequences and in many protein families.
Using four different data sets, we show that DIALIGN is able correctly to align conserved motifs in protein sequences. Alignments produced by DIALIGN are compared systematically to the results of five other alignment programs.
DIALIGN is available to the scientific community free of charge for non-commercial use. Executables for various UNIX platforms including LINUX can be downloaded at http://www.gsf.de/biodv/dialign.html
werner, morgenstern@gsf.de
DIALIGN是一种用于核酸和蛋白质序列的两两比对以及多序列比对的新方法。标准比对程序依赖于比较单个残基并施加空位罚分，而DIALIGN通过比较序列的整个片段来构建比对。不采用空位罚分。如果序列不是全局相关的，而只是共享局部相似性，例如基因组DNA序列和许多蛋白质家族的情况，这种观点就特别适用。
使用四个不同的数据集，我们表明DIALIGN能够正确比对蛋白质序列中的保守基序。将DIALIGN产生的比对结果与其他五个比对程序的结果进行了系统比较。
DIALIGN可供科学界免费用于非商业用途。可以从http://www.gsf.de/biodv/dialign.html下载包括LINUX在内的各种UNIX平台的可执行文件。
werner, morgenstern@gsf.de
