Straif S C, Mbogo C N, Toure A M, Walker E D, Kaufman M, Toure Y T, Beier J C
Department of Tropical Medicine, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA 70112, USA.
J Med Entomol. 1998 May;35(3):222-6. doi: 10.1093/jmedent/35.3.222.
Field studies in Kenya and Mali investigated the prevalence of bacteria in the midguts of malaria vectors, and the potential relationship between gram-negative bacteria species and Plasmodium falciparum sporozoites. Midguts were dissected from 2,430 mosquitoes: 863 Anopheles funestus Giles and 1,037 An. gambiae s.l. Giles from Kenya, and 530 An. gambiae s.l. from Mali. An. funestus had a higher prevalence of gram-negative bacteria (28.5%) compared with An. gambiae collected in Kenya and Mali (15.4 and 12.5%, respectively). Twenty different genera of bacteria were identified by gas chromatography from 73 bacterial isolates from mosquito midguts. Pantoea agglomerans (Enterobacter agglomerans) was the most common species identified. There was no association between gram-negative bacteria in the midgut and P. falciparum sporozoites in field-collected An. gambiae s.l. and An. funestus. However, An. funestus females that harbored gram positive bacteria were more likely to be infected with sporozoites compared with those with no cultivable bacteria or gram negative bacteria in their midguts. Habitat-related variation in the prevalence of diverse types of bacteria in mosquitoes could influence malaria parasite development in mosquitoes and corresponding sporozoite prevalence.
在肯尼亚和马里开展的实地研究调查了疟疾病媒中肠内细菌的流行情况，以及革兰氏阴性菌物种与恶性疟原虫子孢子之间的潜在关系。从2430只蚊子中解剖出中肠：863只来自肯尼亚的费氏按蚊（Anopheles funestus Giles）和1037只冈比亚按蚊复合组（An. gambiae s.l. Giles），以及530只来自马里的冈比亚按蚊复合组。与在肯尼亚和马里采集的冈比亚按蚊（分别为15.4%和12.5%）相比，费氏按蚊的革兰氏阴性菌流行率更高（28.5%）。通过气相色谱法从73株来自蚊子中肠的细菌分离物中鉴定出20个不同的细菌属。成团泛菌（聚团肠杆菌）是鉴定出的最常见物种。在野外采集的冈比亚按蚊复合组和费氏按蚊中，中肠内的革兰氏阴性菌与恶性疟原虫子孢子之间没有关联。然而，与中肠内没有可培养细菌或革兰氏阴性菌的雌性费氏按蚊相比，携带革兰氏阳性菌的雌性费氏按蚊感染子孢子的可能性更大。蚊子中不同类型细菌流行率的栖息地相关差异可能会影响疟原虫在蚊子体内的发育以及相应的子孢子流行率。
