Anezaki K, Asakura H, Honma T, Ishizuka K, Funakoshi K, Tsukada Y, Narisawa R
Third Department of Internal Medicine, Niigata University School of Medicine.
Intern Med. 1998 Mar;37(3):253-8. doi: 10.2169/internalmedicine.37.253.
Interleukin-8 (IL-8) is a peptide which induces not only chemotaxis of neutrophils but also the release of reactive oxygen metabolites from the neutrophils. There are few reports which clarify the relationships between IL-8 and mucosal infiltration of neutrophils or reactive oxygen metabolites produced by neutrophils in the colonic mucosa of ulcerative colitis (UC). Biopsy specimens of colonic mucosa obtained from 26 patients with active UC and 21 patients with inactive UC were studied in order to clarify the relationships among the inflammation factors in UC. Levels of IL-8 and myeloperoxidase in organ culture media of the biopsy specimens from active UC (measured by ELISA and EIA) were significantly higher than those from inactive UC and controls. Reactive oxygen metabolites of biopsy specimens in active UC (measured by luminol-dependent chemiluminescence) were also markedly increased compared to those in inactive UC and controls. The levels of IL-8 were closely correlated to luminol-dependent chemiluminescence or myeloperoxidase levels. However, the levels of IL-8 and myeloperoxidase did not correlate with the grades of activity on colonoendoscopic findings. These findings suggest that IL-8 may play a role in the pathophysiology of UC but it does not define the endoscopic activity grades of UC.
白细胞介素-8（IL-8）是一种不仅能诱导中性粒细胞趋化，还能促使中性粒细胞释放活性氧代谢产物的肽。关于IL-8与溃疡性结肠炎（UC）结肠黏膜中中性粒细胞的黏膜浸润或中性粒细胞产生的活性氧代谢产物之间关系的报道较少。为了阐明UC炎症因子之间的关系，研究人员对26例活动期UC患者和21例非活动期UC患者的结肠黏膜活检标本进行了研究。活动期UC活检标本器官培养基中IL-8和髓过氧化物酶的水平（通过酶联免疫吸附测定法和酶免疫测定法测量）显著高于非活动期UC患者和对照组。与非活动期UC患者和对照组相比，活动期UC活检标本的活性氧代谢产物（通过鲁米诺依赖性化学发光法测量）也明显增加。IL-8水平与鲁米诺依赖性化学发光或髓过氧化物酶水平密切相关。然而，IL-8和髓过氧化物酶水平与结肠镜检查结果的活动度分级无关。这些发现表明，IL-8可能在UC的病理生理学中起作用，但它不能确定UC的内镜活动度分级。
