一种基于编码O157脂多糖的rfb基因座的针对大肠杆菌O157的聚合酶链式反应（PCR）。
A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide.
作者信息
Desmarchelier P M, Bilge S S, Fegan N, Mills L, Vary J C, Tarr P I
机构信息
Commonwealth Scientific and Industrial Research Organisation, Brisbane, Australia.
出版信息
J Clin Microbiol. 1998 Jun;36(6):1801-4. doi: 10.1128/JCM.36.6.1801-1804.1998.
Abstract
A PCR was developed for the detection of Escherichia coli O157 based on the rfbE O-antigen synthesis genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU following crude DNA extraction. The PCR detected < 1 CFU of E. coli O157 per ml in raw milk following enrichment.
摘要
基于rfbE O抗原合成基因开发了一种用于检测大肠杆菌O157的聚合酶链反应（PCR）。在粗DNA提取后，从含有200个或2个菌落形成单位（CFU）的细胞裂解物中的大肠杆菌O157中特异性扩增出479碱基对的PCR产物。富集后，该PCR能检测出生牛奶中每毫升小于1个CFU的大肠杆菌O157。
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