Gupta S, Aggarwal S, Rashanravan B, Lee T
Department of Medicine, University of California, Irvine 92697-4075, USA.
J Neuroimmunol. 1998 May 1;85(1):106-9. doi: 10.1016/s0165-5728(98)00021-6.
Th1-like (IL-2, IFN-gamma) and Th2-like (IL-4, IL-6, and IL-10) cytokines were examined in CD4+ and CD8+ T cells in children with autism. Intracellular cytokines were measured using specific antibodies to various cytokines and anti-CD4 or anti-CD8 monoclonal antibodies by FACScan. Proportions of IFN-gamma+CD4+ T cells and IL-2+CD4+ T cells (Th1), and IFN-gamma+CD8+ and IL-2+CD8+ T cells (TC1) were significantly lower in autistic children as compared to healthy controls. In contrast, IL-4+CD4+ T cells (Th2) and IL-4+CD8+ T cells (TC2) were significantly increased in autism. The proportions of IL-6+ CD4+, IL-6+CD8+ and IL-10+CD4+, IL-10+CD8+ T cells were comparable in autism and control group. These data suggest that an imbalance of Th1- and Th2-like cytokines in autism may play a role in the pathogenesis of autism.
对自闭症患儿的CD4+和CD8+ T细胞中的Th1样（IL-2、IFN-γ）和Th2样（IL-4、IL-6和IL-10）细胞因子进行了检测。通过FACScan使用针对各种细胞因子的特异性抗体以及抗CD4或抗CD8单克隆抗体来测量细胞内细胞因子。与健康对照组相比，自闭症患儿中IFN-γ+CD4+ T细胞和IL-2+CD4+ T细胞（Th1）以及IFN-γ+CD8+和IL-2+CD8+ T细胞（TC1）的比例显著降低。相反，自闭症患儿中IL-4+CD4+ T细胞（Th2）和IL-4+CD8+ T细胞（TC2）显著增加。自闭症组和对照组中IL-6+ CD4+、IL-6+CD8+以及IL-10+CD4+、IL-10+CD8+ T细胞的比例相当。这些数据表明，自闭症中Th1样和Th2样细胞因子的失衡可能在自闭症的发病机制中起作用。
