Park Y I, Shu S, Ramaswamy S B, Srinivasan A
Department of Entomology and Plant Pathology, Mississippi State University, USA.
Arch Insect Biochem Physiol. 1998;38(2):100-7. doi: 10.1002/(SICI)1520-6327(1998)38:2<100::AID-ARCH6>3.0.CO;2-X.
Studies were undertaken to determine whether adult males of Heliothis virescens transfer juvenile hormone (JH) to females during copulation, and an in vitro radiochemical assay was used to determine whether mating causes an allatotropic effect, i.e., stimulation of JH biosynthesis by corpora allata (CA). In vitro, CA from 3-day-old mated females synthesized and released approximately 2.5 times total JH as that of CA from comparably aged virgin females. Of the homologues, JH II exhibited significant increase in mated females; JH I also increased but not significantly. JH III remained similar to that of virgin females. This is the first demonstration of an allatotropic effect of mating in moths. In contrast to the female, CA of virgin males did not produce any JH, but accessory sex glands (ASG) in 3-day-old males synthesized small amounts of JH. Immediately after adult emergence, male ASG contained approximately 1.5 ng JH I and II, which increased by 12 h after emergence and remained at this high level up to 54 h after emergence. JH III was barely detected in ASG. JH in ASG of mated male immediately after uncoupling was depleted almost completely, and 24 h later recovered to levels comparable to that of 54-h-old virgin male. Virgin female bursa copulatrix did not contain any JH, but mated female bursa, immediately after uncoupling, had JH at levels comparable to that observed in virgin male ASG. By 6 h after uncoupling, JH levels decreased dramatically in mated female bursa. These data suggest the transfer of JH to females by the male.
开展了多项研究以确定棉铃虫成年雄虫在交配过程中是否会将保幼激素（JH）传递给雌虫，并且使用体外放射化学分析来确定交配是否会产生促咽侧体效应，即咽侧体（CA）对JH生物合成的刺激作用。在体外，3日龄已交配雌虫的咽侧体合成并释放的JH总量约为同龄未交配雌虫咽侧体的2.5倍。在同系物中，已交配雌虫体内的JH II显著增加；JH I也有所增加，但不显著。JH III与未交配雌虫的水平相似。这是首次证明蛾类交配具有促咽侧体效应。与雌虫不同，未交配雄虫的咽侧体不产生任何JH，但3日龄雄虫的附腺（ASG）会合成少量JH。刚羽化后，雄虫附腺中含有约1.5 ng的JH I和II，羽化后12小时增加，并在羽化后54小时内一直保持在这一高水平。在附腺中几乎检测不到JH III。交配后立即分离的雄虫附腺中的JH几乎完全耗尽，24小时后恢复到与54小时龄未交配雄虫相当的水平。未交配雌虫的交配囊不含任何JH，但交配后立即分离的雌虫交配囊中的JH水平与未交配雄虫附腺中的水平相当。分离后6小时，交配雌虫交配囊中的JH水平急剧下降。这些数据表明雄虫会将JH传递给雌虫。
