芳基硫酸酯酶A和B对两种合成底物的比较活性。
Comparative activity of arylsulphatases A and B on two synthetic substrates.
作者信息
Delvin E E, Pottier A, Glorieux F
出版信息
Biochem J. 1976 Aug 1;157(2):353-6. doi: 10.1042/bj1570353.
Abstract
Rat liver and human skin fibroblasts arylsulphatase A and B activities on both 4-methylumbelliferyl sulphate and 4-nitrocatechol sulphate were compared. The intracellular distribution of activity differed markedly when 4-methylumbelliferyl sulphate was used from that observed with 4-nitrocatechol sulphate. No discrimination between control and metachromatic leucodystrophy or mucopolysaccharidosis (type VI) could be achieved when 4-methylumbelliferyl sulphate was used as substrate. These results contrast sharply with those obtained with 4-nitrocatechol sulphate and cast doubt on the validity of 4-methylumbelliferyl sulphate as substrate for the determination of arylsulphatase A and B activities.
摘要
比较了大鼠肝脏和人皮肤成纤维细胞中芳基硫酸酯酶A和B对4-甲基伞形酮基硫酸盐和4-硝基儿茶酚硫酸盐的活性。当使用4-甲基伞形酮基硫酸盐时，活性的细胞内分布与使用4-硝基儿茶酚硫酸盐时观察到的情况明显不同。当使用4-甲基伞形酮基硫酸盐作为底物时，无法区分对照与异染性脑白质营养不良或黏多糖贮积症（VI型）。这些结果与使用4-硝基儿茶酚硫酸盐获得的结果形成鲜明对比，并对4-甲基伞形酮基硫酸盐作为测定芳基硫酸酯酶A和B活性的底物的有效性提出质疑。
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