异亮氨酸：具有相关编码序列的RNA位点。
Isoleucine:RNA sites with associated coding sequences.
作者信息
Majerfeld I, Yarus M
机构信息
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347, USA.
出版信息
RNA. 1998 Apr;4(4):471-8.
An RNA family that binds isoleucine with Kd = 200-500 microM was repetitively isolated from a ribonucleotide transcript pool containing 50 randomized positions. The RNA site is specific, discriminating against branched side chains of different size (valine--one methylene smaller than isoleucine) by at least 1.3 kcal/mol and against the shape (linear) of norleucine by 0.6 kcal/mol. The binding site was localized by sequence comparison, by synthesis of mutant and truncated active derivatives, and by chemical modification-interference. The binding site is small, no more than 10-12 nt, containing an asymmetric internal loop (6 over 2 nt) that includes the isoleucine codon AUU and a sequence of four G's, two of which are involved in G-U and G-C base pairs. Areas of U/G concentration like these may signal a hydrophobic RNA site.
从一个含有50个随机位置的核糖核苷酸转录本库中反复分离出一个与异亮氨酸结合的RNA家族，其解离常数（Kd）为200 - 500微摩尔。该RNA位点具有特异性，对不同大小的支链侧链（缬氨酸——比异亮氨酸少一个亚甲基）的区分能力至少为1.3千卡/摩尔，对正亮氨酸的形状（线性）的区分能力为0.6千卡/摩尔。通过序列比较、突变体和截短活性衍生物的合成以及化学修饰干扰来确定结合位点。结合位点很小，不超过10 - 12个核苷酸，包含一个不对称内环（6个核苷酸跨越2个核苷酸），其中包括异亮氨酸密码子AUU和四个G的序列，其中两个G参与G - U和G - C碱基对。像这样的U/G富集区域可能表明存在一个疏水性的RNA位点。
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