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连接酶链反应检测法对其他部位感染的女性阴道拭子中沙眼衣原体的检测敏感性。

Sensitivity of the ligase chain reaction assay for detecting Chlamydia trachomatis in vaginal swabs from women who are infected at other sites.

作者信息

Thomas B J, Pierpoint T, Taylor-Robinson D, Renton A M

机构信息

Department of Genitourinary Medicine, Imperial College School of Medicine, St. Mary's, London.

出版信息

Sex Transm Infect. 1998 Apr;74(2):140-1. doi: 10.1136/sti.74.2.140.

DOI:10.1136/sti.74.2.140
PMID:9634328
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1758101/
Abstract

OBJECTIVE

To assess the sensitivity of the ligase chain reaction (LCR) assay for Chlamydia trachomatis in vaginal swabs from women who were positive in cervical samples and/or urines.

SUBJECTS

413 women attending the genitourinary medicine clinic, St. Mary's Hospital, Paddington.

METHODS

The LCR assay was used to test vaginal swabs from 46 women who were C trachomatis positive at one or both of the other sites by direct fluorescent antibody (DFA) staining, by an enzyme immunoassay (EIA), or by the LCR assay.

RESULTS

The LCR assay of vaginal swabs had the following sensitivity values using confirmed positive results: 93% (41/44) compared with DFA staining of cervical deposits, 93% (41/44) compared with the LCR assay of cervical samples, 93% (28/30) compared with an EIA for cervical samples, 91% (39/43) compared with DFA staining of urine deposits, and 93% (39/42) compared with the LCR assay of urine. Four women had vaginal swab samples negative by the LCR assay; one was positive only in the urine and two had cervical samples containing a small number of chlamydial elementary bodies.

CONCLUSION

Testing vaginal swabs by the LCR assay is a sensitive method of detecting chlamydial infection; the results suggest that this procedure could be used as an alternative to examining urines in a screening programme for chlamydial infection in the community.

摘要

目的

评估连接酶链反应（LCR）检测法对宫颈样本和/或尿液检测呈阳性的女性阴道拭子中沙眼衣原体的检测敏感性。

研究对象

413名在帕丁顿圣玛丽医院泌尿生殖医学门诊就诊的女性。

方法

采用LCR检测法对46名女性的阴道拭子进行检测，这些女性在其他一个或两个部位通过直接荧光抗体（DFA）染色、酶免疫测定（EIA）或LCR检测法检测出沙眼衣原体呈阳性。

结果

使用已确认的阳性结果，阴道拭子的LCR检测法具有以下敏感性值：与宫颈沉积物的DFA染色相比为93%（41/44），与宫颈样本的LCR检测法相比为93%（41/44），与宫颈样本的EIA相比为93%（28/30），与尿液沉积物的DFA染色相比为91%（39/43），与尿液的LCR检测法相比为93%（39/42）。4名女性的阴道拭子样本经LCR检测法呈阴性；1名仅尿液呈阳性，2名宫颈样本含有少量衣原体原体。

结论

采用LCR检测法检测阴道拭子是检测衣原体感染的一种敏感方法；结果表明，在社区衣原体感染筛查项目中，该方法可作为尿液检测的替代方法。

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本文引用的文献

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Sensitivity of ligase chain reaction assay of urine from pregnant women for Chlamydia trachomatis.孕妇尿液沙眼衣原体连接酶链反应检测的敏感性
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Chlamydia trachomatis in women: the more you look, the more you find.女性沙眼衣原体感染：越筛查，发现越多。
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