Campo P, Lataye R, Cossec B, Villette V, Roure M, Barthelemy C
Institut National de Recherche et de Sécurité, Laboratoire Multinuisances, Vandoeuvre, France.
Neurotoxicol Teratol. 1998 May-Jun;20(3):321-32. doi: 10.1016/s0892-0362(97)00093-7.
Three experimental groups and one control group of Long-Evans rats were used to study the combined effects of toluene and ethanol on auditory function. The first experimental group was exposed to toluene vapors (1750 ppm, 6 h/day, 5 days/week, 4 weeks), the second one was daily gavaged with a saline solution of ethanol (4 g/kg, 4 weeks), and the last group was simultaneously exposed to both toluene and ethanol. Auditory function was tested by recording brain stem (inferior colliculus) auditory-evoked potentials for audiometric frequencies ranging from 2 to 32 kHz. Urinary hippuric acid was dosed to check the toluene metabolism during the experiments. Ethanol clearly modified the toluene metabolism in the present experimental conditions. As a result, the hearing loss induced by a simultaneous exposure to both ethanol and toluene was larger than that induced by exposure to toluene alone.
使用三组实验组和一组对照组的长 Evans 大鼠来研究甲苯和乙醇对听觉功能的联合影响。第一组实验组暴露于甲苯蒸汽（1750 ppm，每天6小时，每周5天，共4周），第二组每天灌胃乙醇生理盐水溶液（4 g/kg，共4周），最后一组同时暴露于甲苯和乙醇。通过记录2至32 kHz听力测定频率的脑干（下丘）听觉诱发电位来测试听觉功能。在实验期间检测尿马尿酸以检查甲苯代谢情况。在本实验条件下，乙醇明显改变了甲苯代谢。结果，同时暴露于乙醇和甲苯所导致的听力损失大于单独暴露于甲苯所导致的听力损失。
