Ramaswamy N K, Behere A G, Nair P M
Eur J Biochem. 1976 Aug 1;67(1):275-82. doi: 10.1111/j.1432-1033.1976.tb10659.x.
Synthesis of chlorophyll always preceded the synthesis of solanine in cold-stored potatoes in the presence of dim light (30--40 lux). Isolated chloroplasts from green peelings were able to fix CO2. The evidence obtained showed a direct reduction of CO2 to formate, which was the primary product of fixation. Apart from this the chloroplasts were capable of incorporating NaH14CO3, [14C]formate, [2-14/C]glycine, [2-14C]pyruvate, [2-14C]acetate, [2-14C]mevalonate and [U-14C]serine into the solanidine moiety of the alkaloid. The intermediates in the pathway between CO2 and acetate have been identified as formate, glycine, serine and pyruvate. The localization of the enzymes involved in the reaction sequence viz. serine hydroxymethyl-transferase, and pyruvic dehydrogenase complex, in the isolated chloroplast has been established.
在弱光（30 - 40勒克斯）条件下冷藏的马铃薯中，叶绿素的合成总是先于茄碱的合成。从绿色外皮分离得到的叶绿体能够固定二氧化碳。所获得的证据表明二氧化碳直接还原为甲酸，这是固定作用的主要产物。除此之外，叶绿体能够将NaH¹⁴CO₃、[¹⁴C]甲酸、[2 - ¹⁴C]甘氨酸、[2 - ¹⁴C]丙酮酸、[2 - ¹⁴C]乙酸、[2 - ¹⁴C]甲羟戊酸和[U - ¹⁴C]丝氨酸掺入生物碱的茄啶部分。二氧化碳与乙酸之间途径中的中间产物已被鉴定为甲酸、甘氨酸、丝氨酸和丙酮酸。已经确定了参与反应序列的酶，即丝氨酸羟甲基转移酶和丙酮酸脱氢酶复合体在分离的叶绿体中的定位。
