Chávez-Olórtegui C, Zanetti V C, Ferreira A P, Minozzo J C, Mangili O C, Gubert I C
Departamento de Patologia Básica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba, Brazil.
Toxicon. 1998 Apr;36(4):563-9. doi: 10.1016/s0041-0101(97)00159-1.
Enzyme linked immunosorbent assays (ELISA) were developed to detect antigens from Loxosceles intermedia spider venom. Hyperimmune horse anti-Loxosceles intermedia IgGs were prepared by immunoaffinity chromatography and used to set up a sandwich-type ELISA. The specificity of the assay was demonstrated by its capacity to correctly discriminate the circulating antigens in mice that were experimentally inoculated with L. intermedia venom from those inoculated with L. gaucho, L. laeta, and Phoneutria nigriventer spider venoms, Tityus serrulatus scorpion venom and Bothrops jararaca, Crotalus durissus terrificus, Lachesis muta muta and Micrurus frontalis snake venoms. Measurable absorbance signals were obtained with 0.8 ng of venom per assay. The ELISA also detected antigens in the sera of patients envenomed by L. intermedia. Therefore, after standardization for clinical use this ELISA may be a valuable tool for clinicians and epidemiologists.
酶联免疫吸附测定（ELISA）被开发用于检测中间游走蛛毒中的抗原。通过免疫亲和层析制备了超免疫马抗中间游走蛛IgG，并用于建立夹心型ELISA。该测定法的特异性通过其正确区分实验接种中间游走蛛毒液的小鼠与接种高乔游走蛛、巴西游走蛛、黑腹栉足蛛毒液、锯齿蝎毒以及巴西矛头蝮、杜氏响尾蛇、巨蝮和矛头蝮蛇毒的小鼠体内循环抗原的能力得以证明。每次测定使用0.8 ng毒液可获得可测量的吸光度信号。该ELISA还检测到了被中间游走蛛咬伤患者血清中的抗原。因此，经过临床使用标准化后，这种ELISA可能会成为临床医生和流行病学家的一项有价值的工具。
