Nakamura Y, Higaki T, Kato H, Kishida F, Nakatsuka I
Sumitomo Chemical Co., Ltd. Environmental Health Science Laboratory, Osaka, Japan.
J Toxicol Sci. 1998 May;23(2):105-11. doi: 10.2131/jts.23.2_105.
Both male and female guinea pigs are widely used in tests to detect the skin-sensitizing potential of new chemicals. The aim of the present study was to investigate the influence of the animal gender on sensitization rates, with the guinea-pig maximization test (GPMT) using OECD recommended positive control sensitizers, and the differences of the density of Langerhans cells in male and female guinea pigs. The sensitization rates of males and females challenged with HCA, MBT or benzocaine gave almost the same results. There were no statistical differences between the mean responses of males and females. These results were confirmed by rechallenge with the OECD recommended positive controls. Furthermore, the density of Langerhans cells in abdominal epidermis in males (1229 +/- 45 cells/mm2) was the same as that in females (1242 +/- 89 cells/mm2). These results indicate that there is no significant influence of guinea-pig gender in the assessment of skin-sensitizing potential using the GPMT.
雄性和雌性豚鼠都被广泛用于检测新化学物质皮肤致敏潜力的试验中。本研究的目的是利用经合组织推荐的阳性对照致敏剂，通过豚鼠最大化试验（GPMT），研究动物性别对致敏率的影响，以及雄性和雌性豚鼠朗格汉斯细胞密度的差异。用六氯环戊二烯（HCA）、巯基苯并噻唑（MBT）或苯佐卡因攻击的雄性和雌性豚鼠的致敏率几乎相同。雄性和雌性的平均反应之间没有统计学差异。经合组织推荐的阳性对照物再次攻击证实了这些结果。此外，雄性豚鼠腹部表皮中朗格汉斯细胞的密度（1229±45个细胞/mm²）与雌性（1242±89个细胞/mm²）相同。这些结果表明，在使用GPMT评估皮肤致敏潜力时，豚鼠性别没有显著影响。
