致病性原生动物阴道毛滴虫中阴道毛滴虫病毒蛋白的特性分析。

Characterization of Trichomonas vaginalis virus proteins in the pathogenic protozoan T. vaginalis.

作者信息

Liu H W, Chu Y D, Tai J H

机构信息

Division of Infectious Diseases, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China.

出版信息

Arch Virol. 1998;143(5):963-70. doi: 10.1007/s007050050345.

DOI:10.1007/s007050050345

PMID:9645201

Abstract

The 4.6-kb double-stranded (ds) RNA of Trichomonas vaginalis virus (TVV)-T1 has been shown to encode two overlapping genes, cap and pol. In this study, a serum for specifically detecting viral cap gene product was raised against a recombinant protein, and sera for specifically detecting pol gene product were raised against synthetic oligopeptides. A 75-kDa major protein and a 160-kDa minor protein were detected by anti-CAP serum in a TVV-T1 sample, indicating that the 75-kDa protein is the viral capsid protein. The 160-kDa protein alone was also detected by two distinct anti-POL sera, indicating that the pol gene is expressed as a CAP-POL fusion protein. These results suggest that the TVV-T1 genome is arranged into a cap-pol organization in a manner similar to that of viruses in family Totiviridae.

摘要

阴道毛滴虫病毒（TVV）-T1的4.6千碱基对双链（ds）RNA已被证明编码两个重叠基因，即帽状结构（cap）基因和聚合酶（pol）基因。在本研究中，针对重组蛋白制备了用于特异性检测病毒帽状结构基因产物的血清，针对合成寡肽制备了用于特异性检测聚合酶基因产物的血清。抗CAP血清在TVV-T1样本中检测到一种75千道尔顿的主要蛋白和一种160千道尔顿的次要蛋白，表明75千道尔顿的蛋白是病毒衣壳蛋白。两种不同的抗POL血清也单独检测到了160千道尔顿的蛋白，表明pol基因表达为一种CAP-POL融合蛋白。这些结果表明，TVV-T1基因组以类似于双链RNA病毒科病毒的方式排列成帽状结构-聚合酶结构。