Santos E A, Niel C, Vianna C O, De Sá C A, Gomes S A
Department of Virology, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.
Acta Virol. 1998 Feb;42(1):27-33.
Serum samples from 56 human immunodeficiency virus type 1 (HIV-1)-infected adult men were analysed for the presence of hepatitis B virus (HBV) serological markers. Two or more samples from each patient, collected over an interval of 1-6 years, were tested. The antibody against HBV core antigen (anti-HBc) prevalence was 79%. Three (5%) patients No. 5, 7, and 9 were chronic carriers of HBV surface antigen (HBsAg). HBV DNAs from serial samples of these three patients and from two HIV-seronegative control patients were characterised after amplification of different genome regions by polymerase chain reaction (PCR). Size and restriction analyses of the PCR products showed that samples from patients No. 7 (with chronic active hepatitis) and 9 (asymptomatic) contained heterogeneous HBV DNA populations. In patient No. 7, HBV DNA contained a precore gene stop codon mutation at nucleotide (nt) 1896. In addition, a deletion in the core gene was found in a sample collected two years after the onset of acquired immunodeficiency syndrome (AIDS). PCR products from serial samples of patient No. 9 indicated a mixture of HBV DNA molecules that were cloned. Sequencing of the pre-S region of the clones and phylogenetic analysis showed that patient No. 9 was superinfected with three HBV populations of distinct origin, all belonging to genotype A. HBV DNA of patient No. 5 (with AIDS) did not present any variability during a 6-year follow-up. Although two of three HIV/HBV coinfected patients harboured heterogeneous HBV DNA populations during the follow-up, no common event with respect to HBV DNA evolution was observed among the coinfected patients.
对56名感染1型人类免疫缺陷病毒（HIV-1）的成年男性的血清样本进行了乙型肝炎病毒（HBV）血清学标志物检测。对每位患者在1至6年的时间间隔内采集的两份或更多份样本进行了检测。抗HBV核心抗原抗体（抗-HBc）的流行率为79%。3名（5%）患者（5号、7号和9号）为HBV表面抗原（HBsAg）慢性携带者。通过聚合酶链反应（PCR）扩增不同基因组区域后，对这3名患者的系列样本以及两名HIV血清阴性对照患者的HBV DNA进行了特征分析。PCR产物的大小和限制性分析表明，7号患者（患有慢性活动性肝炎）和9号患者（无症状）的样本中含有异质性HBV DNA群体。在7号患者中，HBV DNA在核苷酸（nt）1896处存在前核心基因终止密码子突变。此外，在获得性免疫缺陷综合征（AIDS）发病两年后采集的一份样本中发现核心基因存在缺失。9号患者系列样本的PCR产物表明存在克隆的HBV DNA分子混合物。对克隆的前S区域进行测序和系统发育分析表明，9号患者被三种不同来源的HBV群体重叠感染，均属于A基因型。5号患者（患有AIDS）的HBV DNA在6年随访期间未出现任何变异。尽管三名HIV/HBV合并感染患者中有两名在随访期间携带异质性HBV DNA群体，但在合并感染患者中未观察到关于HBV DNA进化的共同事件。
