Schröder A R, Baumstark T, Riesner D
Institut für Physikalische Biologie, Heinrich-Heine-Universität Düsseldorf, D-40225 Düsseldorf, Germany.
Nucleic Acids Res. 1998 Jul 15;26(14):3449-50. doi: 10.1093/nar/26.14.3449.
In many cases RNA can assume co-existing or meta-stable structures preventing structure determination by chemical mapping. A novel method is described, by which RNA is modified with dimethyl sulphate without shifting the distribution of different structures. The different structures are then separated in native gel electrophoresis, and structure determination by primer extension can be carried out separately for each structure.
在许多情况下，RNA可呈现共存或亚稳态结构，从而阻碍通过化学图谱法确定其结构。本文描述了一种新方法，利用该方法用硫酸二甲酯修饰RNA，而不改变不同结构的分布。然后通过非变性凝胶电泳分离不同结构，并可对每种结构分别进行引物延伸法结构测定。
