醋酸纤维素凝胶上乙二醛酶I（GLO）的快速检测及荷兰人群中GLO变体的分布
Rapid detection of glyoxalase I (GLO) on cellulose acetate gel and the distribution of GLO variants in a Dutch population.
作者信息
Meera Khan P, Doppert B A
出版信息
Hum Genet. 1976 Sep 10;34(1):53-6. doi: 10.1007/BF00284434.
PMID:965004
Abstract
A rapid electrophoretic procedure is described for detecting the human red cell glyoxalase I variants (GLO 1, GLO 2-1, and GLO 2) on cellulose acetate gel (cellogel) on which the sites of enzymed activity are visualized as purple bands against white background. The frequency of GLO1 gene in a Dutch population living in and around Leiden was found to be 0.4544.
摘要
本文描述了一种快速电泳方法，用于在醋酸纤维素凝胶（cellogel）上检测人类红细胞乙二醛酶I变体（GLO 1、GLO 2-1和GLO 2），在该凝胶上酶活性位点在白色背景下呈现为紫色条带。研究发现，生活在莱顿及其周边地区的荷兰人群中GLO1基因的频率为0.4544。
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