Eklund M W, Poysky F T, Peterson M E, Meyers J A
Infect Immun. 1976 Sep;14(3):793-803. doi: 10.1128/iai.14.3.793-803.1976.
The relationship of specific bacteriophages to the production of the lethal alpha toxin in Clostridium novyi types A and B was investigated. When type A strain 5771 reverted to the phage-sensitive state, it ceased to produce alpha toxin but continued to produce the gamma and epsilon antigens. This "nontoxigenic" culture, therefore, more closely resembled C. botulinum types C and D than the other C. novyi types. Phage-sensitive type B strains also ceased to produce the alpha toxin but continued to produce the beta toxin, and therefore very colesly resembled C. novyi type D (C. haemolyticum). Alpha toxin was again produced when the phage-sensitive cultures were reinfected with the respective tox+ phages. Alpha toxin production could also be induced in the "nontoxigenic" phage-sensitive derivatives from type B strain 8024 by tox+ phages isolated from other strains of type B. tox- phages were also isolated, but they did not affect alpha toxin production. The tox+ phages also caused a marked change in the colonial morphology of type B strains. In this report we present evidence that alpha toxin production by C. novyi type A strain 5771 and type B strain 8024 depends upon the continued presence and participation of specific bacteriophages designated as NA1tox+ and NB1tox+, respectively.
研究了特定噬菌体与诺维氏梭菌A和B型中致死性α毒素产生之间的关系。当A型菌株5771回复到噬菌体敏感状态时，它停止产生α毒素，但继续产生γ和ε抗原。因此，这种“无毒素产生”的培养物与肉毒梭菌C型和D型的相似性比与其他诺维氏梭菌类型的相似性更高。噬菌体敏感的B型菌株也停止产生α毒素，但继续产生β毒素，因此与诺维氏梭菌D型（溶血梭菌）非常相似。当噬菌体敏感培养物用相应的产毒素噬菌体重新感染时，又会产生α毒素。从B型其他菌株分离出的产毒素噬菌体也能在B型菌株8024的“无毒素产生”噬菌体敏感衍生物中诱导产生α毒素。也分离出了不产毒素的噬菌体，但它们不影响α毒素的产生。产毒素噬菌体还引起了B型菌株菌落形态的明显变化。在本报告中，我们提供证据表明，诺维氏梭菌A型菌株5771和B型菌株8024产生α毒素分别依赖于分别命名为NA1tox +和NB1tox +的特定噬菌体的持续存在和参与。
