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通过聚合酶链反应（PCR）检测培养阳性支气管肺泡灌洗液中嗜肺军团菌的样本制备方法比较

Comparison of sample preparation methods for detection of Legionella pneumophila in culture-positive bronchoalveolar lavage fluids by PCR.

作者信息

Jaulhac B, Reyrolle M, Sodahlon Y K, Jarraud S, Kubina M, Monteil H, Piémont Y, Etienne J

机构信息

Institut de Bactériologie de la Faculté de Médecine, Université Louis Pasteur et Hopitaux Universitaires de Strasbourg, France.

出版信息

J Clin Microbiol. 1998 Jul;36(7):2120-2. doi: 10.1128/JCM.36.7.2120-2122.1998.

DOI:10.1128/JCM.36.7.2120-2122.1998
PMID:9650980
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC104998/
Abstract

A prospective study was conducted on 25 Legionella pneumophila culture-positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-based method (P = 0.03). N difference was found concerning the inhibition rate.

摘要

对25份嗜肺军团菌培养阳性和98份培养阴性的支气管肺泡灌洗液样本进行了一项前瞻性研究，以比较两种DNA制备方法：一种基于改良Chelex的快速方案和一种蛋白酶K方法。发现基于Chelex的方法进行PCR检测时更敏感（P = 0.03）。在抑制率方面未发现差异。

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本文引用的文献

1
Diagnosis of Legionella pneumophila, Mycoplasma pneumoniae, or Chlamydia pneumoniae lower respiratory infection using the polymerase chain reaction on a single throat swab specimen.使用聚合酶链反应对单一咽拭子标本进行嗜肺军团菌、肺炎支原体或肺炎衣原体下呼吸道感染的诊断。
Diagn Microbiol Infect Dis. 1996 Jan;24(1):7-14. doi: 10.1016/0732-8893(95)00254-5.
2
Use of polymerase chain reaction in an epidemiologic investigation of Pontiac fever.聚合酶链反应在庞蒂亚克热流行病学调查中的应用。
J Infect Dis. 1993 Sep;168(3):769-72. doi: 10.1093/infdis/168.3.769.
3
Legionnaires' disease.军团病
Clin Infect Dis. 1993 Jun;16(6):741-7. doi: 10.1093/clind/16.6.741.
4
Rapid detection of Legionella species in bronchoalveolar lavage fluids with the EnviroAmp Legionella PCR amplification and detection kit.使用EnviroAmp军团菌PCR扩增检测试剂盒快速检测支气管肺泡灌洗液中的军团菌属。
J Clin Microbiol. 1993 Dec;31(12):3325-8. doi: 10.1128/jcm.31.12.3325-3328.1993.
5
Evaluation of commercial amplification kit for detection of Legionella pneumophila in clinical specimens.用于检测临床标本中嗜肺军团菌的商用扩增试剂盒的评估
J Clin Microbiol. 1994 Jun;32(6):1503-5. doi: 10.1128/jcm.32.6.1503-1505.1994.
6
Construction of a DNA amplification assay for detection of Legionella species in clinical samples.用于检测临床样本中军团菌属的DNA扩增检测方法的构建。
Eur J Clin Microbiol Infect Dis. 1994 Mar;13(3):225-31. doi: 10.1007/BF01974541.
7
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J Clin Microbiol. 1994 Dec;32(12):3068-9. doi: 10.1128/jcm.32.12.3068-3069.1994.
8
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J Clin Microbiol. 1994 Oct;32(10):2616-9. doi: 10.1128/jcm.32.10.2616-2619.1994.
9
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Eur J Clin Microbiol Infect Dis. 1995 Jan;14(1):25-33. doi: 10.1007/BF02112614.
10
Reevaluation of the definition of Legionnaires' disease: use of the urinary antigen assay. Community Based Pneumonia Incidence Study Group.军团病定义的重新评估：尿抗原检测的应用。社区肺炎发病率研究组。
Clin Infect Dis. 1995 May;20(5):1286-91. doi: 10.1093/clinids/20.5.1286.