应用ABC-ELISA法检测囊尾蚴病抗体的猪囊尾蚴培养基抗原的研究

A study on the culture medium antigens of Cystcercus cellulosae for detecting antibodies of cysticercosis by means of ABC-ELISA.

作者信息

Guo H, Zhao Z F, Shi D Z

机构信息

Department of Parasitology, Hainan Medical College, Haikou, People's Republe of China.

出版信息

Southeast Asian J Trop Med Public Health. 1997;28 Suppl 1:125-7.

PMID:9656362

Abstract

Two antigens of Cysticercus cellulosae, cystic fluid antigen (CFA) and the culture medium antigen (CMA), were used in Avidin-Biotin Peroxdase Complex-ELISA (ABC-ELISA) to detect IgG antibodies in 45 cases of cysticercosis treated with praziquantel. The results revealed the total positive rates as 51.11% with CMA and 82.22% with CFA. The positive rates in the cases treated within 2 courses of treatment were 79.17% for CMA and 87.50% for CFA, and only 19.05% for CMA and 71.43% for CFA in the cases treated for more then 3 courses. The fact that the positive rates decreased as the courses of treatment increased showed that the sensitivity of CMA might be related to the vital conditions of the worms in the body, whether alive or dead. It is, therefore, recommended that CMA has the potential to be employed in ABC-ELISA both as an indicator for diagnosing cysticercosis and as a reference for the evaluation of the treatment.

摘要

应用猪囊尾蚴的两种抗原，即囊液抗原（CFA）和培养液抗原（CMA），采用抗生物素蛋白-生物素过氧化物酶复合物酶联免疫吸附测定法（ABC-ELISA）检测45例经吡喹酮治疗的囊尾蚴病患者的IgG抗体。结果显示，CMA的总阳性率为51.11%，CFA的总阳性率为82.22%。在接受2个疗程以内治疗的病例中，CMA的阳性率为79.17%，CFA的阳性率为87.50%；而在接受3个疗程以上治疗的病例中，CMA的阳性率仅为19.05%，CFA的阳性率为71.43%。阳性率随治疗疗程增加而下降这一事实表明，CMA的敏感性可能与体内虫体的存活状态有关。因此，建议CMA有潜力在ABC-ELISA中用于诊断囊尾蚴病及评估治疗效果的参考指标。