对粪便样本进行筛查以鉴定耐万古霉素肠球菌分离株时，应包括甲基-α-D-吡喃葡萄糖苷试验，以区分不运动的鹑鸡肠球菌和粪肠球菌。
Screening of stool samples for identification of vancomycin-resistant Enterococcus isolates should include the methyl-alpha-D-glucopyranoside test to differentiate nonmotile Enterococcus gallinarum from E. faecium.
作者信息
Turenne C Y, Hoban D J, Karlowsky J A, Zhanel G G, Kabani A M
机构信息
Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
出版信息
J Clin Microbiol. 1998 Aug;36(8):2333-5. doi: 10.1128/JCM.36.8.2333-2335.1998.
Abstract
The methyl-alpha-D-glucopyranoside (MDG) test has been shown to be superior to motility testing in differentiating Enterococcus faecium from E. gallinarum. In the present study, 33 vancomycin-resistant enterococcus (VRE) isolates collected as part of a stool surveillance study were compared by using motility and MDG. Motility testing identified all 33 isolates as E. faecium, whereas MDG identified 11 of the 33 isolates as nonmotile E. gallinarum. The MDG results were confirmed by sequencing the 16S rDNA V6-to-V8 region. We conclude that the MDG test is a necessary component of routine VRE screening.
摘要
已证明甲基-α-D-吡喃葡萄糖苷（MDG）试验在区分粪肠球菌和鹑鸡肠球菌方面优于动力试验。在本研究中，对作为粪便监测研究一部分收集的33株耐万古霉素肠球菌（VRE）分离株进行了动力试验和MDG试验比较。动力试验将所有33株分离株鉴定为粪肠球菌，而MDG试验将33株分离株中的11株鉴定为无动力的鹑鸡肠球菌。通过对16S rDNA V6至V8区域进行测序，证实了MDG试验结果。我们得出结论，MDG试验是常规VRE筛查的必要组成部分。
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