Hanson K L, Cartwright C P
Microbiology Department, Clinical Laboratories, Hennepin County Medical Center, Minneapolis, Minnesota 55415, USA.
J Clin Microbiol. 1999 Mar;37(3):815-7. doi: 10.1128/JCM.37.3.815-817.1999.
Three different methodologies, reduction of litmus milk (LM) and acidification of arabinose (ARA), acidification of methyl-alpha-D-glucopyranoside (MGP), and rapid motility (RM), for differentiating isolates of Enterococcus casseliflavus and Enterococcus gallinarum (intrinsically vancomycin-resistant enterococci [IVRE]) from Enterococcus faecalis and Enterococcus faecium were evaluated. All 33 isolates of E. faecalis tested reduced LM within 4 h and were negative in all other tests, while the 53 isolates of E. faecium were ARA positive only. In contrast, 45 of 46 (98%) IVRE isolates examined (26 E. casseliflavus and 20 E. gallinarum isolates) acidified MGP, 41 of 46 (89%) were LM and ARA positive, and 45 of 46 (98%) were RM positive. Acidification of MGP was therefore the single most useful test for differentiating IVRE from vancomycin-resistant E. faecium and E. faecalis; however, a combination of LM-ARA and RM testing enabled the correct designation of organisms without the need for overnight incubation.
评估了三种不同的方法，即石蕊牛奶（LM）还原、阿拉伯糖（ARA）酸化、甲基-α-D-吡喃葡萄糖苷（MGP）酸化和快速运动性（RM），用于区分格氏肠球菌和鹑鸡肠球菌（固有耐万古霉素肠球菌[IVRE]）与粪肠球菌和屎肠球菌的分离株。所有测试的33株粪肠球菌分离株在4小时内还原了LM，在所有其他测试中均为阴性，而53株屎肠球菌分离株仅ARA呈阳性。相比之下，在检测的46株（98%）IVRE分离株中（26株格氏肠球菌和20株鹑鸡肠球菌分离株），45株使MGP酸化，46株中有41株（89%）LM和ARA呈阳性，46株中有45株（98%）RM呈阳性。因此，MGP酸化是区分IVRE与耐万古霉素屎肠球菌和粪肠球菌的最有用的单一测试；然而，LM-ARA和RM测试的组合能够正确鉴定菌株，无需过夜培养。
