McLaren C, Ellis M N, Hunter G A
Antiviral Res. 1983 Nov;3(4):223-34. doi: 10.1016/0166-3542(83)90001-3.
A quantitative colorimetric method for measuring the inhibition of viral cytopathic effects has been adapted to the assay of antiviral compounds. Drug-treated, virus-infected cultures in microtiter plates were stained with the vital dye neutral red and the amount of dye incorporated was determined in a multichannel spectrophotometer. The technique required smaller volumes of reagents, was more easily automated than the standard plaque reduction assay and had good reproducibility. Standard conditions of 30 infectious units of challenge virus and 72-h incubation were judged to be optimal. Median inhibitory concentrations (ID50) for a number of compounds were approximately tenfold higher in the dye-uptake assay compared with the plaque reduction assay, possibly related to the higher multiplicity of infection required to give the desired level of cytopathic effect in the microtiter method.
一种用于测量病毒细胞病变效应抑制作用的定量比色法已被应用于抗病毒化合物的检测。在微量滴定板中，用活性染料中性红对经药物处理且感染病毒的培养物进行染色，并在多通道分光光度计中测定染料掺入量。该技术所需试剂体积更小，比标准蚀斑减少试验更易于自动化，且具有良好的重现性。30个感染单位的攻击病毒和72小时孵育的标准条件被判定为最佳。与蚀斑减少试验相比，许多化合物在染料摄取试验中的半数抑制浓度（ID50）大约高10倍，这可能与在微量滴定法中产生所需细胞病变效应水平所需的更高感染复数有关。
