布基纳法索锡德拉杜古地区感染采采蝇的锥虫的PCR分析及空间分布

PCR analysis and spatial repartition of trypanosomes infecting tsetse flies in Sidéradougou area of Burkina Faso.

作者信息

De La Rocque S, Lefrancois T, Reifenberg J M, Solano P, Kabore I, Bengaly Z, Augusseau X, Cuisance D

机构信息

CIRDES, 01 BP 454, Burkina Faso.

出版信息

Ann N Y Acad Sci. 1998 Jun 29;849:32-8. doi: 10.1111/j.1749-6632.1998.tb11030.x.

DOI:10.1111/j.1749-6632.1998.tb11030.x

PMID:9668447

Abstract

A parasitological and entomological survey was conducted in the Sideradougou area (south of Bobo Dioulasso, Burkina Faso) in order to identify transmission factors of African trypanosomosis. A total of 3600 tsetse flies (Glossina tachinoides, Glossina palpalis gambiensis) were captured along 120 km of linear gallery forest and half of them were dissected. PCR analysis was undertaken on parasitologically positive flies (161 G. tachinoides, 92 G. palpalis gambiensis) to characterize the different trypanosomes. All the results were integrated in a GIS (Geographical Information System). Spatial repartition of the characterized trypanosomes enabled to recognize different areas with specific patterns of infection. Relations with environmental factors are discussed.

摘要

在锡德拉杜古地区（布基纳法索博博迪乌拉索以南）开展了一项寄生虫学和昆虫学调查，以确定非洲锥虫病的传播因素。沿着120公里的线状长廊林共捕获了3600只采采蝇（刺舌蝇、冈比亚须舌蝇），并解剖了其中一半。对寄生虫学检测呈阳性的采采蝇（161只刺舌蝇、92只冈比亚须舌蝇）进行PCR分析，以鉴定不同的锥虫。所有结果都整合到了一个地理信息系统（GIS）中。已鉴定锥虫的空间分布使得能够识别出具有特定感染模式的不同区域。文中还讨论了与环境因素的关系。