Sung M K, Lautens M, Thompson L U
Department of Nutritional Sciences, University of Toronto, Ont., Canada.
Anticancer Res. 1998 May-Jun;18(3A):1405-8.
The mammalian lignans enterolactone (EL) and enterodiol (ED) derived from precursors in foods, particularly flaxseed, have been shown to reduce the mammary tumor growth due to their antiestrogenic properties. Our objective was to investigate whether lignans have growth inhibitory effects against colon tumor cells and whether these effects are mediated through their antiestrogenic activity.
Four human colon tumor cell lines (LS174T, Caco-2, HCT-15, T-84) were incubated with various levels of EL, ED or 17 beta-estradiol for 8 to 10 days. Cell proliferation was measured by the BrdU method.
At 100 microM concentration, both lignans significantly reduced the proliferation of all cell lines. EL was more than twice as effective as ED at this concentration. The growth was not affected by the presence of 17 beta-estradiol implying that these cells are not estrogen-sensitive.
Lignans are growth inhibitors of colon tumor cells and they may act through mechanism(s) other than antiestrogenic activity.
源自食物（尤其是亚麻籽）中前体物质的哺乳动物木脂素肠内酯（EL）和肠二醇（ED），因其抗雌激素特性已被证明可抑制乳腺肿瘤生长。我们的目的是研究木脂素对结肠肿瘤细胞是否具有生长抑制作用，以及这些作用是否通过其抗雌激素活性介导。
将四种人结肠肿瘤细胞系（LS174T、Caco - 2、HCT - 15、T - 84）与不同浓度的EL、ED或17β - 雌二醇孵育8至10天。通过BrdU法测量细胞增殖。
在100微摩尔浓度下，两种木脂素均显著降低了所有细胞系的增殖。在此浓度下，EL的效果是ED的两倍多。17β - 雌二醇的存在对生长没有影响，这意味着这些细胞对雌激素不敏感。
木脂素是结肠肿瘤细胞的生长抑制剂，它们可能通过抗雌激素活性以外的机制起作用。
