Madden R H, Moran L, Scates P
Food Science Department (Food Microbiology), Department of Agriculture for Northern Ireland, Belfast, Northern Ireland.
J Appl Microbiol. 1998 May;84(5):703-8. doi: 10.1046/j.1365-2672.1998.00397.x.
Sampling of lamb (n = 100) and beef (n = 100) carcasses in abattoirs in Northern Ireland produced no evidence of Campylobacter spp. contamination and when retail packs of beef (n = 50) and pork (n = 50) were sampled these were also apparently free of Campylobacter spp. However, 38% of retail packs of chicken pieces (n = 120), yielded Campylobacter spp. These packs were purchased over a period of 1 year and came from a single local producer. After the species of the isolates had been determined (Campylobacter jejuni and Camp. coli were found in approximately equal numbers) they were subtyped using both polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the random amplified polymorphic DNA (RAPD) method of typing. All of the poultry isolates were successfully typed by these methods, in contrast to the results obtained with serotyping where several isolates were found to be untypable. PCR-RFLP typing showed that specific subtypes were isolated repeatedly over a period of 1 year in the output of the producer studied. The more discriminating RAPD confirmed this observation, but with fewer isolates. This appears to indicate recurrent infection of broilers whose source can now be investigated using the methodologies developed.
在北爱尔兰的屠宰场对100只羔羊和100头牛的胴体进行采样，未发现弯曲杆菌属污染的迹象。对50份零售牛肉包装和50份零售猪肉包装进行采样时，这些包装显然也没有弯曲杆菌属。然而，120份零售鸡块包装中有38%检测出弯曲杆菌属。这些包装是在1年的时间内购买的，均来自当地的一家生产商。在确定分离菌株的种类后（空肠弯曲杆菌和结肠弯曲杆菌的数量大致相等），使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和随机扩增多态性DNA（RAPD）分型方法对其进行亚型分析。与血清分型的结果相反，所有家禽分离株都成功地通过这些方法进行了分型，血清分型中有几个分离株无法分型。PCR-RFLP分型显示，在研究的生产商的产品中，特定的亚型在1年的时间内反复出现。更具鉴别力的RAPD证实了这一观察结果，但分离株较少。这似乎表明肉鸡反复感染，现在可以使用所开发的方法对其来源进行调查。
