通过脱氧核糖核酸酶I消化揭示，在尿素中展开的染色质中十核苷酸重复序列的持久性。
Persistence of the ten-nucleotide repeat in chromatin unfolded in urea, as revealed by digestion with deoxyribonuclease i.
作者信息
Yaneva M, Dessev G
出版信息
Nucleic Acids Res. 1976 Jul;3(7):1761-7. doi: 10.1093/nar/3.7.1761.
Abstract
It is shown by enzymatic digestion of chromatin from rat liver or Guerin ascites tumour (GAT) that treatments, which abolish the 180 base pair repeat, as revealed by digestion with micrococcal nuclease (shearing in salt solutions of medium ionic strength, sonication, fixation with formaldehyde in the presence of 5 M urea), have little effect on the 10 nucleotide repeat, observed in deoxyribonuclease I digests.
摘要
通过对大鼠肝脏或格林腹水瘤（GAT）的染色质进行酶消化表明，那些消除180碱基对重复序列的处理（如用微球菌核酸酶消化所揭示的，在中等离子强度的盐溶液中剪切、超声处理、在5M尿素存在下用甲醛固定），对在脱氧核糖核酸酶I消化中观察到的10核苷酸重复序列几乎没有影响。
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