Ghim S Y, Choi S K, Shin B S, Jeong Y M, Sorokin A, Ehrlich S D, Park S H
Bacterial Molecular Genetics RU, Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon.
DNA Res. 1998 Jun 30;5(3):195-201. doi: 10.1093/dnares/5.3.195.
The nucleotide sequence of 45,389 bp in the 184 degrees-180 degrees region of the Bacillus subtilis chromosome, containing the cge cluster, which is controlled by the sporulation regulatory protein GerE, was determined. Fifty-four putative ORFs with putative ribosome-binding sites were recognized. Seven of them correspond to previously characterized genes: cgeB, cgeA, cgeC, cgeD, cgeE, ctpA, and odhA. The deduced products of 25 ORFs were found to display significant similarities to proteins in the data banks. We have identified genes involved in detoxification, cell walls, and in the metabolism of biotins, purines, fatty acids, carbohydrates and amino acids. The remaining 22 ORFs showed no similarity to known proteins. Both an attachment site of the SPbeta prophage and 2 new putative DNA replication terminators were identified in this region.
测定了枯草芽孢杆菌染色体184°-180°区域中45389 bp的核苷酸序列，该区域包含受芽孢形成调节蛋白GerE控制的cge簇。识别出54个具有假定核糖体结合位点的假定开放阅读框（ORF）。其中7个对应于先前已鉴定的基因：cgeB、cgeA、cgeC、cgeD、cgeE、ctpA和odhA。发现25个ORF的推导产物与数据库中的蛋白质具有显著相似性。我们鉴定出了参与解毒、细胞壁以及生物素、嘌呤、脂肪酸、碳水化合物和氨基酸代谢的基因。其余22个ORF与已知蛋白质没有相似性。在该区域鉴定出了SPβ原噬菌体的一个附着位点和2个新的假定DNA复制终止子。
