Senyuta N, Syrtsev A, Yamashita M, Stepina V, Susova O, Scherbak L, Pavlish O, Hayami M, Gurtsevitch V
Cancer Research Center, RAMS, Moscow, Russia.
Int J Cancer. 1998 Aug 12;77(4):488-93. doi: 10.1002/(sici)1097-0215(19980812)77:4<488::aid-ijc2>3.0.co;2-q.
Phylogenetic analysis of HTLV-I variants persisting among population of Turkmenistan and the Republic of Georgia (situated on both sides of the Caspian Sea, not far from a known HTLV-I cluster in Northern Iran) has been carried out. HTLV-I isolates from the above countries were obtained in the course of seroepidemiological investigations. In all, 1,510 blood-donor samples from Turkmenistan and 47 blood samples from hematological patients from Georgia, were tested with different screening and confirmatory assays, including commercial ones. As a result, 7 infected individuals (3 blood donors and 4 relatives of one blood donor) from Turkmenistan and 3 infected individuals (one ATL patient and 2 of his relatives) from Georgia were found. In addition, 4 HTLV-I isolates from Turkmenistan and one from Georgia were cloned and part of their LTR was sequenced. Phylogenetic analysis of sequenced isolates allowed us to conclude that these isolates belonged to sub-type A, one of 3 sub-types of the "Cosmopolitan" type. We found that most of the Turkmenian isolates formed a tight cluster and shared common nucleotide substitutions with isolates originating from Northern Iran (Mashhad). The data obtained suggest that there is a new endemic focus of HTLV-I infection in the Caspian Sea region. Further investigations are required to confirm this hypothesis.
对土库曼斯坦和格鲁吉亚（位于里海两岸，距离伊朗北部已知的HTLV-I聚集区不远）人群中持续存在的HTLV-I变异体进行了系统发育分析。上述国家的HTLV-I分离株是在血清流行病学调查过程中获得的。总共对来自土库曼斯坦的1510份献血者样本和来自格鲁吉亚血液学患者的47份血液样本进行了不同的筛查和确证检测，包括商业检测。结果，在土库曼斯坦发现了7名感染者（3名献血者和1名献血者的4名亲属），在格鲁吉亚发现了3名感染者（1名成人T细胞白血病患者及其2名亲属）。此外，对来自土库曼斯坦的4株HTLV-I分离株和来自格鲁吉亚的1株进行了克隆，并对其部分长末端重复序列（LTR）进行了测序。对测序分离株的系统发育分析使我们得出结论，这些分离株属于A亚型，是“世界型”的3个亚型之一。我们发现，大多数土库曼斯坦分离株形成了一个紧密的聚类，并与来自伊朗北部（马什哈德）的分离株共享常见的核苷酸替换。获得的数据表明，里海地区存在一个新的HTLV-I感染地方病疫源地。需要进一步调查以证实这一假设。
