Benekli M, Güllü I H, Tekuzman G, Savaş M C, Hayran M, Hasçelik G, Firat D
Institute of Oncology, Hacettepe University School of Medicine, Ankara, Turkey.
Br J Cancer. 1998 Jul;78(2):267-71. doi: 10.1038/bjc.1998.476.
A diversity of adhesive interactions occur between the cancer cell and host extracellular matrix which potentiate neoplastic expansion and metastatic dissemination. In miscellaneous malignant diseases, tumour progression has been observed to be associated with alterations in adhesion molecule expression. Recently, circulating soluble intercellular adhesion molecules have been identified. In this study, serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) and soluble E-selectin (sE-selectin) were determined in patients with gastric cancer. The study group consisted of 27 patients with previously untreated gastric adenocarcinoma. Four patients had stage II, two patients stage III and 21 patients stage IV disease according to the TNM classification. Nineteen patients had distant metastasis. The sera obtained from 18 healthy volunteers served as controls. Serum sICAM-1 and sE-selectin concentrations were determined by enzyme-linked immunosorbent assay (ELISA). In addition, we also studied other tumour-associated antigens, i.e. CEA and CA 19-9. Serum sICAM-1 levels were significantly increased in patients with gastric cancer (P < 0.0001). However, sE-selectin levels did not differ from the controls. sICAM-1 concentrations were also significantly higher in patients with distant metastasis and peritoneal spread (P = 0.0045 and P = 0.0157 respectively), whereas sE-Selectin levels were elevated only in patients with peritoneal metastasis (P = 0.033). Serum concentrations of sICAM-1 and sE-selectin correlated with CEA levels (P = 0.0013 and P = 0.003 respectively). Elevated levels of sE-selectin were associated with poorer prognosis (P = 0.0099), whereas sICAM-1 had no significant impact on survival. Our results suggest that increased sICAM-1 serum levels may reflect widespread disease and contribute directly to the progression of gastric cancer. Further investigation of the molecular mechanisms of adhesive tumour-host interactions may lead to a better understanding of the natural history of gastric cancer.
癌细胞与宿主细胞外基质之间存在多种黏附相互作用，这些作用会促进肿瘤的扩张和转移扩散。在各种恶性疾病中，已观察到肿瘤进展与黏附分子表达的改变有关。最近，循环可溶性细胞间黏附分子已被鉴定出来。在本研究中，测定了胃癌患者血清中可溶性细胞间黏附分子-1（sICAM-1）和可溶性E-选择素（sE-选择素）的水平。研究组由27例未经治疗的胃腺癌患者组成。根据TNM分类，4例患者为II期，2例患者为III期，21例患者为IV期疾病。19例患者有远处转移。从18名健康志愿者获得的血清用作对照。通过酶联免疫吸附测定（ELISA）测定血清sICAM-1和sE-选择素浓度。此外，我们还研究了其他肿瘤相关抗原，即癌胚抗原（CEA）和糖类抗原19-9（CA 19-9）。胃癌患者血清sICAM-1水平显著升高（P < 0.0001）。然而，sE-选择素水平与对照组无差异。远处转移和腹膜播散患者的sICAM-1浓度也显著更高（分别为P = 0.0045和P = 0.0157），而sE-选择素水平仅在腹膜转移患者中升高（P = 0.033）。血清sICAM-1和sE-选择素浓度与CEA水平相关（分别为P = 0.0013和P = 0.003）。sE-选择素水平升高与预后较差相关（P = 0.0099），而sICAM-1对生存率无显著影响。我们的结果表明，血清sICAM-1水平升高可能反映疾病广泛存在，并直接促进胃癌的进展。对黏附性肿瘤-宿主相互作用分子机制的进一步研究可能有助于更好地理解胃癌的自然病程。
