Matsuda M, Asami Y, Miyazawa T, Samata T, Isayama Y, Honda M, Ide Y
Department of Biosciences, Azabu University, Sagamihara, Japan.
Vet Res Commun. 1994;18(2):93-8. doi: 10.1007/BF01839225.
Analysis of chromosome-sized DNA and genome typing of Taylorella equigenitalis NCTC11184, Kentucky 188, and five strains of T. equigenitalis isolated in Japan were carried out. The three restriction enzymes used, ApaI, NaeI and NotI, cleaved the genomic DNAs of five Japanese strains of T. equigenitalis into relatively limited numbers of restriction fragments, which were well resolved on crossed-field gel electrophoresis (CFGE). The respective profiles after CFGE of the restriction fragments from all five strains were essentially identical to each other after digestion by ApaI, NaeI or NotI. Hence it appears that these strains have a common genome type with respect to these three restriction enzymes. It was also shown that the respective profiles from these strains were essentially different from those of T. equigenitalis NCTC11184 and those of Kentucky 188 after digestion with ApaI, NaeI or NotI.
对马生殖道泰勒菌NCTC11184、肯塔基188以及在日本分离出的五株马生殖道泰勒菌进行了染色体大小DNA分析和基因组分型。所使用的三种限制性内切酶ApaI、NaeI和NotI，将五株日本马生殖道泰勒菌的基因组DNA切割成相对数量有限的限制性片段，这些片段在正交场凝胶电泳（CFGE）上得到了很好的分离。在用ApaI、NaeI或NotI消化后，所有五株菌的限制性片段在CFGE后的各自图谱基本彼此相同。因此，就这三种限制性内切酶而言，这些菌株似乎具有共同的基因组类型。还表明，在用ApaI、NaeI或NotI消化后，这些菌株的各自图谱与马生殖道泰勒菌NCTC11184以及肯塔基188的图谱基本不同。
