Matuschka F R, Schinkel T W, Klug B, Spielman A, Richter D
Institut für Pathologie, Charité, Medizinische Fakultät der Humboldt-Universität zu Berlin, 12249 Berlin, Germany.
Appl Environ Microbiol. 1998 Aug;64(8):3089-91. doi: 10.1128/AEM.64.8.3089-3091.1998.
To define conditions promoting inherited infection by Lyme disease spirochetes in Ixodes ticks, we variously infected ticks with Borrelia afzelii and examined their progenies by dark-field microscopy, immunofluorescence, PCR, and serial passage. No episode of inherited infection was evident, regardless of instar or gender infected or frequency of exposure. We suggest that these spirochetes rarely, if ever, are inherited by vector ticks.
为了确定促进莱姆病螺旋体在硬蜱中发生遗传性感染的条件,我们用阿氏疏螺旋体对蜱进行了不同方式的感染,并通过暗视野显微镜检查、免疫荧光、聚合酶链反应(PCR)和连续传代对其后代进行了检测。无论受感染的蜱虫龄期或性别如何,也无论接触频率怎样,均未发现明显的遗传性感染情况。我们认为,这些螺旋体极少(如果有的话)通过媒介蜱进行遗传。