Nakamura N, Miyazawa C, Sawada S, Akiyama M, Awa A A
Department of Genetics, Radiation Effects Research Foundation, Hiroshima, Japan.
Int J Radiat Biol. 1998 Jun;73(6):619-27. doi: 10.1080/095530098141870.
To estimate gamma-ray doses received by Hiroshima atomic bomb survivors using electron spin resonance (ESR) of tooth enamel and to compare the results with cytogenetic data.
Tooth enamel ESR was performed for 100 teeth donated from 69 Hiroshima survivors, and conventional cytogenetic examinations were conducted for 61 of the tooth donors. To evaluate possible contamination from dental X-ray exposure, which primarily affects the tooth's buccal surface, each tooth was divided into buccal and lingual parts for subsequent independent enamel isolation and ESR measurement.
Almost 20 teeth showed considerably larger buccal doses than lingual doses, but most of these discrepant teeth were incisors and canines. The results are probably attributable to solar light exposure. In contrast, the buccal and lingual doses found in molars were similar. Conventional translocation data of lymphocytes and ESR-estimated doses of 40 donors of molars showed the dose-response for translocations to be almost the same as that expected from in vitro gamma-ray irradiation experiments.
Both tooth enamel ESR and lymphocyte cytogenetics are useful measures for individual biodosimetry of acute radiation exposure, even half a century after the exposure occurred.
利用牙釉质的电子自旋共振（ESR）估算广岛原子弹幸存者所接受的γ射线剂量，并将结果与细胞遗传学数据进行比较。
对69名广岛幸存者捐赠的100颗牙齿进行牙釉质ESR检测，对其中61名牙齿捐赠者进行常规细胞遗传学检查。为评估主要影响牙齿颊面的牙科X射线照射可能造成的污染，将每颗牙齿分为颊面和舌面部分，随后分别独立分离牙釉质并进行ESR测量。
近20颗牙齿的颊面剂量明显高于舌面剂量，但这些差异较大的牙齿大多为门牙和犬齿。结果可能归因于阳光照射。相比之下，臼齿的颊面和舌面剂量相似。40名臼齿捐赠者的淋巴细胞常规易位数据和ESR估算剂量显示，易位的剂量反应与体外γ射线照射实验预期的几乎相同。
即使在辐射暴露发生半个世纪后，牙釉质ESR和淋巴细胞细胞遗传学都是急性辐射暴露个体生物剂量测定的有用方法。
