Prall Y G, Gambhir K K, Ampy F R
Department of Medicine, Howard University, Washington, DC 20059, USA.
Life Sci. 1998;63(3):177-84. doi: 10.1016/s0024-3205(98)00258-6.
The purpose of this investigation was to determine whether acetylcholinesterase (AChE) can be used as a marker of cell aging in human red blood cells (RBCs). This study used consented subjects; both males and females in an age range of 21-42 years. The blood samples (8-9 mL) were drawn in tubes containing sodium heparin or EDTA as anticoagulants. To avoid contamination with other cells, (lymphocytes, monocytes and reticulocytes), RBCs were purified (PRBC) by Hypaque-Ficoll gradient technique. The PRBCs were subfractionated into young (y) (1.08-1.09), mid (m) (1.09-1.11) and old (o) (1.11-1.12) percoll density (g/mL) fractions using a discontinuous percoll gradient. The mean +/- 1 SD AChE per gram hemoglobin (U/g Hgb) activities in whole blood (WB) purified human red blood cells (PRBCs), young human red blood cells (y-RBCs), mid age human red blood cells (m-RBCs) and old human red blood cells (o-RBCs) were 27.4 +/- 2.98, 26.0 +/- 2.33, 25.5 +/- 1.64, 20.3 +/- 3.84, 14.6 +/- 3.42 in males and 26.3 +/- 4.44, 24.8 /- 4.83, 26.4 +/- 4.59, 24.0 +/- 5.50 and 12.4 +/- 7.09 in females respectively. Although there was variation in the data, the results indicated that old human red blood cells showed significantly (p<.05) lower AChE activity compared to young human red blood cells of both sexes. These preliminary but novel observations suggest that AChE can be an excellent enzymatic marker for RBC aging in man.
本研究的目的是确定乙酰胆碱酯酶（AChE）是否可作为人类红细胞（RBC）细胞衰老的标志物。本研究使用了已签署知情同意书的受试者，年龄在21至42岁之间的男性和女性。将8 - 9毫升血液样本采集到含有肝素钠或乙二胺四乙酸（EDTA）作为抗凝剂的试管中。为避免与其他细胞（淋巴细胞、单核细胞和网织红细胞）污染，通过聚蔗糖 - 泛影葡胺梯度技术纯化红细胞（PRBC）。使用不连续的聚蔗糖梯度将PRBC亚分级为年轻（y）（1.08 - 1.09）、中年（m）（1.09 - 1.11）和老年（o）（1.11 - 1.12）聚蔗糖密度（克/毫升）级分。男性全血（WB）、纯化的人类红细胞（PRBC）、年轻人类红细胞（y - RBCs）、中年人类红细胞（m - RBCs）和老年人类红细胞（o - RBCs）中每克血红蛋白（U/g Hgb）的平均±1标准差AChE活性分别为27.4±2.98、26.0±2.33、25.5±1.64、20.3±3.84、14.6±3.42，女性分别为26.3±4.44、24.8±4.83、26.4±4.59、24.0±5.50和12.4±7.09。尽管数据存在差异，但结果表明，与两性的年轻人类红细胞相比，老年人类红细胞的AChE活性显著降低（p<0.05）。这些初步但新颖的数据表明，AChE可能是人类红细胞衰老的一个优秀酶标志物。
