Maney T, Hunter A W, Wagenbach M, Wordeman L
Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington 98195, USA.
J Cell Biol. 1998 Aug 10;142(3):787-801. doi: 10.1083/jcb.142.3.787.
Mitotic centromere-associated kinesin (MCAK) is recruited to the centromere at prophase and remains centromere associated until after telophase. MCAK is a homodimer that is encoded by a single gene and has no associated subunits. A motorless version of MCAK that binds centromeres but not microtubules disrupts chromosome segregation during anaphase. Antisense-induced depletion of MCAK results in the same defect. MCAK overexpression induces centromere-independent bundling and eventual loss of spindle microtubule polymer suggesting that centromere-associated bundling and/or depolymerization activity is required for anaphase. Live cell imaging indicates that MCAK may be required to coordinate the onset of sister centromere separation.
有丝分裂着丝粒相关驱动蛋白（MCAK）在前期被招募到着丝粒，并一直与着丝粒结合，直到末期之后。MCAK是一种同型二聚体，由单个基因编码，没有相关亚基。一种不具有运动功能但能结合着丝粒而不结合微管的MCAK版本会在后期破坏染色体分离。反义诱导的MCAK缺失会导致相同的缺陷。MCAK的过表达会诱导不依赖着丝粒的微管束集以及纺锤体微管聚合物最终丧失，这表明后期需要着丝粒相关的束集和/或解聚活性。活细胞成像表明，可能需要MCAK来协调姐妹着丝粒分离的起始。
