Koh J Y, Cho N P, Kong G, Lee J D, Yoon K
Department of Oral Pathology and Institute of Dental Science, School of Dentistry, Chonbuk National University, Chonju, Korea.
Br J Cancer. 1998 Aug;78(3):354-9. doi: 10.1038/bjc.1998.498.
Forty-two oral squamous cell carcinomas (SCCs) were analysed for p53 mutations and human papillomavirus (HPV) infection to examine the prevalency of these factors and correlation with apoptotic index (AI; number of apoptotic cells per 100 tumour cells) of the tumour tissue. In polymerase chain reaction (PCR)-Southern blot analysis, HPV DNAs were detected from 22 out of 42 SCCs (52%) with predominance of HPV-16 (68%). p53 mutations in exons 5-8, screened by nested PCR-single-strand conformation polymorphism (PCR-SSCP) analysis, were observed in 16 of 42 tumours (38%). The state of the p53 gene did not show any correlation with HPV infection. The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labelling (TUNEL) method was used for detection of apoptotic cells. The mean AI was 2.35, ranging from 0.31 to 6.63. SCCs associated with p53 mutation had significantly lower AI than those without p53 mutation (P < 0.01), whereas no difference in AI was found between SCCs with and without HPV infection. The results of this study confirmed that HPV infection and/or p53 mutations are implicated, but are not mutually exclusive events, in carcinogenesis of oral SCC and also showed that decrease in apoptosis is more closely related to p53 mutation than HPV infection.
对42例口腔鳞状细胞癌（SCC）进行p53突变和人乳头瘤病毒（HPV）感染分析，以检测这些因素的发生率及其与肿瘤组织凋亡指数（AI；每100个肿瘤细胞中的凋亡细胞数）的相关性。在聚合酶链反应（PCR）-Southern印迹分析中，42例SCC中有22例（52%）检测到HPV DNA，其中以HPV-16为主（68%）。通过巢式PCR-单链构象多态性（PCR-SSCP）分析筛查外显子5-8中的p53突变，42例肿瘤中有16例（38%）观察到该突变。p53基因状态与HPV感染无相关性。采用末端脱氧核苷酸转移酶（TdT）介导的dUTP-生物素缺口末端标记（TUNEL）法检测凋亡细胞。平均AI为2.35，范围为0.31至6.63。与无p53突变的SCC相比，伴有p53突变的SCC的AI显著降低（P<0.01），而HPV感染阳性和阴性的SCC之间的AI无差异。本研究结果证实，HPV感染和/或p53突变与口腔SCC的致癌作用有关，但并非相互排斥事件，并且还表明凋亡减少与p53突变的关系比与HPV感染的关系更为密切。
