Benoit-Vical F, Valentin A, Cournac V, Pélissier Y, Mallié M, Bastide J M
Laboratoire d'Immunologie et Parasitologie, Faculté de Pharmacie, Montpellier, France.
J Ethnopharmacol. 1998 Jul;61(3):173-8. doi: 10.1016/s0378-8741(98)00036-1.
Aqueous extracts from Nauclea latifolia S.M. (Rubiaceae), a plant commonly used in Ivory Coast by traditional healers for the treatment of malaria, were tested on two strains of Plasmodium faliparum: FcB1-Colombia (chloroquine-resistant) and a Nigerian strain (chloroquine-sensitive). The extracts were obtained from stems and roots of the plant in two forms, infusion and decoction, both methods used by most traditional healers. The in vitro activity of N. latifolia extracts on P. falciparum was assessed both visually and by a radioactive method. The visual analysis allowed determination of the time of extract action on the erythrocytic cycle, as well as the parasitic stage of most inhibitory effect. Similar results were obtained applying fresh, frozen or lyophilized extracts. The IC50 values determined were within the range already reported for other antimalarial plants such as Azadirachta indica A. Juss (Meliaceae) or Artemisia annua L. (Asteraceae). Aqueous extracts of N. latifolia inhibited P. falciparum (FcB1 strain) mainly at the end of the erythrocytic cycle (32nd to 48th hour).
阔叶乌檀（茜草科）是科特迪瓦传统治疗师常用于治疗疟疾的一种植物，其水提取物在两种恶性疟原虫菌株上进行了测试：FcB1 - 哥伦比亚株（耐氯喹）和一种尼日利亚株（氯喹敏感）。提取物从该植物的茎和根以两种形式获得，即浸泡液和煎剂，这两种方法都是大多数传统治疗师所使用的。阔叶乌檀提取物对恶性疟原虫的体外活性通过视觉和放射性方法进行评估。视觉分析可以确定提取物对红细胞周期的作用时间，以及产生最大抑制作用的寄生虫阶段。使用新鲜、冷冻或冻干提取物获得了相似的结果。所测定的半数抑制浓度（IC50）值在已报道的其他抗疟植物如印楝（楝科）或青蒿（菊科）的范围内。阔叶乌檀水提取物主要在红细胞周期末期（第32至48小时）抑制恶性疟原虫（FcB1株）。
