Benoit F, Valentin A, Pélissier Y, Marion C, Dakuyo Z, Mallié M, Bastide J M
Laboratoire d'Immunologie et Parasitologie, Faculté de Pharmacie, UFR Sciences Pharmaceutiques, Montpellier, France.
Trans R Soc Trop Med Hyg. 1995 Mar-Apr;89(2):217-8. doi: 10.1016/0035-9203(95)90502-2.
Resistance of Plasmodium falciparum to current antimalarial compounds has drastically increased during the last few years and is now a major public health problem. We have studied plants traditionally used in Africa against malaria. Extracts of the tubercles of Cochlospermum tinctorium A. Rich, commonly used in Burkina Faso, were tested in vitro on 2 strains of P. falciparum, one (FcB1-Colombia) chloroquine resistant and the other (F32-Tanzania) chloroquine sensitive. Extracts were obtained by infusion and decoction. The 50% inhibitory concentrations (IC50) were determined by measuring [3H]hypoxanthine incorporation and also by microscopical examination which permitted the determination of parasite stages. We obtained similar results with fresh extracts, frozen extracts, and lyophilized extracts of C. tinctorum. IC50 values were of the order of 1-2 micrograms/mL, about one-tenth of those reported for extracts of neem leaves (Azadirachta indica) and about half the values reported for Artemisia annua extracts.
在过去几年中，恶性疟原虫对现有抗疟化合物的耐药性急剧增加，现已成为一个主要的公共卫生问题。我们研究了非洲传统上用于治疗疟疾的植物。对布基纳法索常用的染色柯桠（Cochlospermum tinctorium A. Rich）块茎提取物进行了体外试验，受试对象为2株恶性疟原虫，一株（FcB1-哥伦比亚株）对氯喹耐药，另一株（F32-坦桑尼亚株）对氯喹敏感。提取物通过浸泡和煎煮获得。通过测量[3H]次黄嘌呤掺入量以及通过显微镜检查（可确定寄生虫阶段）来测定50%抑制浓度（IC50）。我们对染色柯桠的新鲜提取物、冷冻提取物和冻干提取物得到了相似的结果。IC50值约为1-2微克/毫升，约为印楝叶（Azadirachta indica）提取物报告值的十分之一，约为青蒿提取物报告值的一半。
