Gibson J R, Slater E, Xerry J, Tompkins D S, Owen R J
Helicobacter Reference Unit, Laboratory of Enteric Pathogens, Central Public Health Laboratory, London NW9 5HT, United Kingdom.
J Clin Microbiol. 1998 Sep;36(9):2580-5. doi: 10.1128/JCM.36.9.2580-2585.1998.
Amplified-fragment length polymorphism (AFLP) analysis is the name given to a genotypic technique in which adapter oligonucleotides are ligated to restriction enzyme fragments and then used as target sites for primers in a PCR amplification process. The amplified fragments are electrophoretically separated to give strain-specific band profiles. We have developed a single-enzyme approach that did not require costly equipment or reagents for the fingerprinting of strains of Helicobacter pylori. The method was assessed with 46 isolates of H. pylori from 28 patients, and the results were compared with those from other genotypic tests. The AFLP profiles derived from HindIII fragments differentiated strains of H. pylori from unrelated individuals and confirmed the common origin of strains in some family members. AFLP analysis was also applied to investigate persistent infection following antibiotic therapy. Overall, the modified technique was relatively rapid and technically simple yet gave reproducible and discriminatory results. AFLP analysis samples variation throughout the genome and is a valuable addition to the existing genotypic fingerprinting methods for H. pylori.
扩增片段长度多态性（AFLP）分析是一种基因型技术，在该技术中，衔接子寡核苷酸与限制性酶切片段连接，然后在PCR扩增过程中用作引物的靶位点。扩增片段通过电泳分离，以给出菌株特异性的条带图谱。我们开发了一种单酶方法，该方法不需要昂贵的设备或试剂即可对幽门螺杆菌菌株进行指纹分析。用来自28名患者的46株幽门螺杆菌分离株对该方法进行了评估，并将结果与其他基因型检测结果进行了比较。源自HindIII片段的AFLP图谱区分了来自无关个体的幽门螺杆菌菌株，并证实了一些家庭成员中菌株的共同起源。AFLP分析还用于研究抗生素治疗后的持续感染。总体而言，改良后的技术相对快速且技术简单，但能给出可重复且具有鉴别力的结果。AFLP分析可检测整个基因组的变异，是现有幽门螺杆菌基因型指纹分析方法的一项有价值的补充。
