Kotronias D, Kapranos N
Department of Obstetrics and Gynaecology, Mitera Maternity and Surgical Center, Athens, Greece.
In Vivo. 1998 Jul-Aug;12(4):391-4.
The role of herpes simplex virus (HSV) in male infertility has been investigated using the localizing ability of in situ hybridization technique. Sperm samples obtained from 80 men attending a maternity center because of fertility problems were classified by spermogram and analyzed for the presence of HSV-1 and HSV-2 DNA using digoxigenin-labelled DNA probes. HSV DNA was detected in the nuclei of spermatozoa in 37 semen samples (46%), HSV-1 specifically in 21 cases (26%) and HSV-2 in 16 cases (20%). HSV infection was almost three times more common in semens with a sperm count lower than 20 million/ml in relation to semens with a sperm count higher than 20 million/ml (70% versus 25.5%, P = 0.0001). The mean sperm count and motility for HSV positive semens were 23.5% and 36% respectively, whereas those of HSV negative semens were 53.2% and 47% respectively (P(count) = 0.0005 and P(motility) = 0.01). The number of HSV positive sperm cells ranged from 2 cells per specimen to 60% of the sperm cells. The mean number of HSV-2 labelled sperm cells per sample was 3.7% and that of HSV-1 1.5%. The percentage of hybridization positive sperm cells was also inversely correlated with sperm count and motility. Acyclovir therapy of eight males with HSV positive semens resulted in three successful pregnancies. In conclusion, HSV seems to play an important role in male infertility and the detection of this virus in the semen will not only allow substantial therapeutic interventions for the restoration of fertility but it will also contribute to the control of the transmission of HSV infection.
利用原位杂交技术的定位能力，对单纯疱疹病毒（HSV）在男性不育中的作用进行了研究。从80名因生育问题前往妇产科中心就诊的男性身上获取精子样本，根据精子图谱进行分类，并使用地高辛标记的DNA探针分析HSV - 1和HSV - 2 DNA的存在情况。在37份精液样本（46%）的精子细胞核中检测到HSV DNA，其中HSV - 1特异性检测到21例（26%），HSV - 2检测到16例（20%）。与精子计数高于2000万/ml的精液相比，精子计数低于2000万/ml的精液中HSV感染的发生率几乎高出三倍（70%对25.5%，P = 0.0001）。HSV阳性精液的平均精子计数和活力分别为23.5%和36%，而HSV阴性精液的平均精子计数和活力分别为53.2%和47%（P（计数）= 0.0005，P（活力）= 0.01）。HSV阳性精子细胞的数量范围为每个样本2个细胞至精子细胞的60%。每个样本中HSV - 2标记的精子细胞的平均数量为3.7%，HSV - 1为1.5%。杂交阳性精子细胞的百分比也与精子计数和活力呈负相关。对8名HSV阳性精液的男性进行阿昔洛韦治疗后，有3例成功怀孕。总之，HSV似乎在男性不育中起重要作用，在精液中检测到这种病毒不仅将允许采取大量治疗干预措施来恢复生育能力，而且还将有助于控制HSV感染的传播。
