Umeda M, Contreras A, Chen C, Bakker I, Slots J
Department of Periodontology, Tokyo Medical and Dental University, Japan.
J Periodontol. 1998 Jul;69(7):828-33. doi: 10.1902/jop.1998.69.7.828.
This study compared the presence of 6 periodontopathic bacteria in whole saliva and subgingival plaque of 202 subjects. The test bacteria were identified using a 16S rRNA-based PCR detection method. Each study subject contributed a whole saliva sample and a paper point sample pooled from the deepest periodontal pocket in each quadrant of the dentition. The kappa test revealed a fair agreement between the presence of Porphyromonas gingivalis, Prevotella intermedia, and Treponema denticola in whole saliva and periodontal pocket samples (kappa > 0.4). The McNemar test showed that the differences between sample types were due to a more frequent detection of the 3 organisms in whole saliva than in periodontal pocket samples (P < 0.01). Prevotella nigrescens also was detected more frequently in whole saliva than in periodontal pocket samples (P < 0.01; McNemar test). Although little agreement between samples was found for Actinobacillus actinomycetemcomitans and Bacteroides forsythus (kappa < or = 0.4), neither whole saliva nor pocket samples showed better detection for these 2 species (P < 0.01, McNemar test). The results indicate that whole saliva is superior to pooled periodontal pocket samples to detect P. gingivalis, P. intermedia, P. nigrescens, and T. denticola in the oral cavity. The detection of oral A. actinomycetemcomitans and B. forsythus with reasonably good accuracy may require both whole saliva and periodontal pocket samples.
本研究比较了202名受试者全唾液和龈下菌斑中6种牙周病原菌的存在情况。使用基于16S rRNA的聚合酶链反应（PCR）检测方法鉴定测试细菌。每位研究对象提供一份全唾液样本和一份从牙列各象限最深牙周袋采集的纸尖样本。kappa检验显示，牙龈卟啉单胞菌、中间普氏菌和具核梭杆菌在全唾液和牙周袋样本中的存在情况有较好的一致性（kappa>0.4）。McNemar检验表明，样本类型之间的差异是由于这3种微生物在全唾液中的检测频率高于牙周袋样本（P<0.01）。变黑普氏菌在全唾液中的检测频率也高于牙周袋样本（P<0.01；McNemar检验）。虽然放线共生放线杆菌和福赛坦氏拟杆菌在样本间的一致性较差（kappa≤0.4），但全唾液和牙周袋样本对这2种菌的检测效果均不佳（P<0.01，McNemar检验）。结果表明，在检测口腔中的牙龈卟啉单胞菌、中间普氏菌、变黑普氏菌和具核梭杆菌方面，全唾液优于混合的牙周袋样本。要以合理的准确度检测口腔中的放线共生放线杆菌和福赛坦氏拟杆菌，可能既需要全唾液样本，也需要牙周袋样本。
