Jung Woo-Ri, Joo Ji-Young, Lee Ju-Youn, Kim Hyun-Joo
Department of Periodontology, Dental and Life Science Institute, Pusan National University School of Dentistry, Yangsan, Korea.
Department of Periodontology, Dental Research Institute, Pusan National University Dental Hospital, Yangsan, Korea.
J Periodontal Implant Sci. 2021 Oct;51(5):316-328. doi: 10.5051/jpis.2006640332.
This study aimed to examine the prevalence and abundance of 9 representative periodontal pathogens in the saliva samples of periodontally healthy subjects (PH) and patients with periodontitis who underwent supportive periodontal therapy (SPT). The age-specific distribution of these pathogens in periodontally healthy individuals was also analyzed.
One hundred subjects (aged >35 years) were recruited (50 each in the PH and SPT groups) between August 2016 and April 2019. The prevalence and abundance of periodontal pathogens in the PH group were compared with those in periodontally healthy young subjects (94 subjects; aged <35 years), who were included in our previous study. DNA copy numbers of (), (), (), (), , (), (), (), and () were analyzed using real-time polymerase chain reaction.
The detection frequencies of all pathogens, except , were high in the PH and SPT groups. The ranking order of pathogen DNA copy numbers was similar in both groups. In both groups, had the highest abundance, had the lowest abundance. Additionally, was significantly more abundant in men than in women in both groups (<0.05). Compared with the PH group, the SPT group exhibited significantly lower total bacteria and abundance and higher abundance (<0.05). The age-specific pathogen distribution analysis revealed a significantly low abundance and high and abundance in the PH group.
The clinical parameters and microbial profiles were similar between the SPT and PH groups. However, patients with periodontitis require supportive care to prevent recurrence. As the abundance of some bacteria varied with age, future studies must elucidate the correlation between age-related physiological changes and periodontal bacterial composition.
本研究旨在检测牙周健康受试者（PH）和接受牙周支持治疗（SPT）的牙周炎患者唾液样本中9种代表性牙周病原体的患病率和丰度。还分析了这些病原体在牙周健康个体中的年龄特异性分布。
在2016年8月至2019年4月期间招募了100名受试者（年龄>35岁）（PH组和SPT组各50名）。将PH组中牙周病原体的患病率和丰度与我们之前研究中纳入的牙周健康年轻受试者（94名；年龄<35岁）进行比较。使用实时聚合酶链反应分析（）、（）、（）、（）、、（）、（）、（）和（）的DNA拷贝数。
除了外，所有病原体在PH组和SPT组中的检测频率都很高。两组中病原体DNA拷贝数的排序相似。在两组中，的丰度最高，的丰度最低。此外，两组中男性的丰度均显著高于女性（<0.05）。与PH组相比，SPT组的总细菌和丰度显著降低，丰度更高（<0.05）。年龄特异性病原体分布分析显示，PH组中的丰度显著较低，和的丰度较高。
SPT组和PH组的临床参数和微生物谱相似。然而，牙周炎患者需要支持性护理以防止复发。由于某些细菌的丰度随年龄变化，未来的研究必须阐明与年龄相关的生理变化与牙周细菌组成之间的相关性。
