Nielsen O L, Jørgensen P H, Hedemand J, Jensenius J C, Koch C, Laursen S B
Danish Veterinary Laboratory, Aarhus, Denmark.
Immunology. 1998 May;94(1):122-8. doi: 10.1046/j.1365-2567.1998.00462.x.
This paper describes the results of immuno-histochemical staining for chicken mannan-binding lectin (MBL) in formalin-fixed tissue sections from non-infected chickens, and from chickens infected with infectious laryngotracheitis virus (ILTV) or infectious bursal disease virus (IBDV). In the non-infected chickens, MBL was detected in the cytoplasm of a few hepatocytes and in the germinal centres of the caecal tonsils, whereas sections of kidney, heart muscle, spleen, cerebrum, thymus, adrenal gland, bursa of Fabricius, bone marrow and trachea were without staining. In the ILTV-infected chickens, an intense staining reaction for MBL was detected in the cytoplasm of all hepatocytes and on the surface of, and inside, ILTV-infected cells. Also in the IBDV-infected chickens, an intense staining reaction for MBL was detected in the cytoplasm of all hepatocytes. No staining was seen in the follicles of the bursa of Fabricius, but MBL was present in non-identified cells in the interstitium, and in the cytoplasm of macrophage-like cells, located peripheral to the ellipsoid of the spleen. These findings indicate the liver as the primary site of MBL synthesis, and points to up-regulation as a result of the viral infections. The location outside the liver could indicate a role of MBL in the immune defence.
本文描述了对未感染鸡、感染传染性喉气管炎病毒（ILTV）或传染性法氏囊病病毒（IBDV）的鸡的福尔马林固定组织切片进行鸡甘露聚糖结合凝集素（MBL）免疫组织化学染色的结果。在未感染的鸡中，在少数肝细胞的细胞质和盲肠扁桃体的生发中心检测到MBL，而肾脏、心肌、脾脏、大脑、胸腺、肾上腺、法氏囊、骨髓和气管的切片未染色。在感染ILTV的鸡中，在所有肝细胞的细胞质以及ILTV感染细胞的表面和内部均检测到MBL的强烈染色反应。同样在感染IBDV的鸡中，在所有肝细胞的细胞质中也检测到MBL的强烈染色反应。在法氏囊的滤泡中未观察到染色，但MBL存在于间质中未鉴定的细胞以及位于脾脏椭球体周围的巨噬细胞样细胞的细胞质中。这些发现表明肝脏是MBL合成的主要部位，并指出病毒感染导致MBL上调。肝脏外的定位可能表明MBL在免疫防御中的作用。
