Fraulin F O, Bahoric A, Harrop A R, Hiruki T, Clarke H M
Department of Surgery, Hospital for Sick Children, Ontario.
J Burn Care Rehabil. 1998 Jul-Aug;19(4):337-45. doi: 10.1097/00004630-199807000-00012.
A new method of delivery of epithelial suspensions with use of an aerosolization apparatus was examined in the pig. Full-thickness pig skin was harvested, and an epithelial suspension was created using standard techniques of dispase and trypsin. Twenty-four hours after skin harvest, four full-thickness wounds were created on the flanks of the pig. The control wound was sprayed with a solution without epithelial cells. The three experimental wounds were sprayed with epithelial cell suspensions (integral 10(6) cells/suspension). Weekly evaluation with photographs, biopsies, and tracings were done for 4 weeks. At 10 weeks, the entire process was repeated with new wounds on the pig's back. Thirty-five wounds in five pigs were evaluated: 10 control (5 flank, 5 back) and 25 experimental (15 flank, 10 back). Control wounds healed by contraction alone, with epithelium at the edges only. After 4 weeks, an open area remained. Central epithelial islands developed in experimental wounds at 2 weeks. These islands coalesced to close the wounds by 4 weeks. Histology at 1 week showed groups of epithelial cells deeply embedded in granulation tissue. These groups became immature epithelial layers on the surface by 2 weeks, and all layers of epithelium were present by 4 weeks. Overall, flank experimental wounds epithelialized sooner, but contracted at the same rate as control wounds. In conclusion, epithelial cells can be delivered by an aerosolization apparatus and remain viable and proliferative in a pig model.
在猪身上研究了一种使用雾化装置递送上皮悬浮液的新方法。获取猪的全层皮肤，采用标准的中性蛋白酶和胰蛋白酶技术制备上皮悬浮液。取皮后24小时，在猪的侧腹制造四个全层伤口。对照伤口喷洒不含上皮细胞的溶液。三个实验伤口喷洒上皮细胞悬浮液（每悬浮液含10⁶个细胞）。连续4周每周通过拍照、活检和绘图进行评估。10周时，在猪背部制造新伤口重复整个过程。对五头猪的35个伤口进行了评估：10个对照伤口（5个在侧腹，5个在背部）和25个实验伤口（15个在侧腹，10个在背部）。对照伤口仅通过收缩愈合，仅边缘有上皮。4周后仍有开放区域。实验伤口在2周时出现中央上皮岛。这些岛在4周时融合以闭合伤口。1周时的组织学显示上皮细胞群深深嵌入肉芽组织。到2周时这些群变成表面的未成熟上皮层，到4周时上皮的所有层均已出现。总体而言，侧腹实验伤口上皮化更快，但收缩速度与对照伤口相同。总之，上皮细胞可通过雾化装置递送，并在猪模型中保持存活和增殖。
